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Course, academic year 2023/2024
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Microinjection and micromanipulation - practical course - MB151C02
Title: Mikroinjekční a mikromanipulační praktikum
Czech title: Mikroinjekční a mikromanipulační praktikum
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2020
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:0/2, C [TS]
Capacity: 20
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Note: enabled for web enrollment
Guarantor: doc. RNDr. Ing. Vladimír Krylov, Ph.D.
Teacher(s): Mgr. Zuzana Burdíková, Ph.D.
doc. RNDr. Ing. Vladimír Krylov, Ph.D.
Mgr. Marie Macůrková, Ph.D.
RNDr. Tereza Tlapáková, Ph.D.
Annotation -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (18.09.2019)
The aim of this practical course is to familiarize students with microinjection and micromanipulation techniques on the model of round worm (C. elegans) and clawed frogs (Xenopus laevis and Xenopus tropicalis). Students will have also the oportunity to work with advanced microscopic systems such as light sheet and live cell immaging microscopy.
Literature -
Last update: RNDr. Nataša Šebková, Ph.D. (24.10.2019)

Literature will be available at each course.

Requirements to the exam -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (26.08.2019)

course attendance, working out the practical course protocols, to be able to communicate in English language.


Syllabus -
Last update: doc. RNDr. Ing. Vladimír Krylov, Ph.D. (18.09.2019)

Task 1: RNA interference of the embryonic lethal gene in C. elegans.


- C. elegans morphology, principle of the RNA interference

- in vitro synthesis of dsRNA

- preparation of microinjection tools (capillary, pads for the C. elegans immobilization)

- familiarization with the micromanipulator, microinjection process


Task 2: Study of migration potential of stem cells


- what are stem cells?

- meet Xenopus!

- preparation of RFP positive Sertoli stem cells derived from the testes of X. tropicalis juvenile male for the microinjection

- preparation of media, capillary and microinjection tools

- microinjection of Sertoli stem cells into X. tropicalis tadpoles

- light sheet microscopy - observation of migration routes of microinjected cells in living tadpoles

- preparation of photodocumentation

The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362

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