Single molecule microscopy and manipulation - practical course - MB151C01E
|
|
Opinion survey results Schedule
|
||
|
Recording a single molecular motor protein walking along a microtubule or following diffusion of proteins in living cells became almost routine tasks, allowing to get insights into molecular interactions of biomolecules. Single molecule microscopy and manipulation practical course focuses on optical based methods of detection, characterization and even manipulation of single molecules. The aim is to highlight the uniqueness of single molecule based methods for uncovering the mechanisms of molecular interactions in biophysics, but also to show participants the real laboratory world of single molecule experiments.
The course covers traditional methods of single molecule observation using TIRF based wide-field microscopy and confocal microscopy combined with spectral and excited state lifetime resolved detection. The single molecule manipulation, including force measurement and confocal imaging of the manipulated molecules, is performed using the optical tweezers. The methodologies covered include single particle tracking and various modifications of fluorescence correlation spectroscopy. Last update: Benda Aleš, Mgr., Ph.D. (15.10.2018)
|
|
||
|
Single-Molecule Techniques: A Laboratory Manual, Cold Spring Harbor Laboratory Press 2008, ISBN 978-087969775-4 Handbook of Single-Molecule Biophysics, Springer New York 2009, ISBN 978-1-4939-3919-0 Last update: Šebková Nataša, RNDr., Ph.D. (06.06.2022)
|
|
||
|
Active participation at the course (max. 1 day of absence - excluding Monday) plus short 3 min presentation of your own work or future plans. Last update: Benda Aleš, Mgr., Ph.D. (07.10.2019)
|
|
||
|
http://web.natur.cuni.cz/IMCF/SMMM/programme.html Last update: Olšinová Marie, Mgr., Ph.D. (09.10.2021)
|
|
||
|
The course is intended for magister and doctoral students. Last update: Benda Aleš, Mgr., Ph.D. (21.09.2017)
|
| The course does not include work placement |