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Course, academic year 2023/2024
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Single molecule microscopy and manipulation - practical course - MB151C01E
Title: Single molecule microscopy and manipulation - practical course
Czech title: Mikroskopie a manipulace jednotlivých molekul – praktický kurz
Guaranteed by: Biology Section (31-101)
Faculty: Faculty of Science
Actual: from 2023
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:0/1, C [TS]
Capacity: 8
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Explanation: Please register first at the webpage of the course.Selected participants will add this course to their list of subjects later.
Additional information:
Note: enabled for web enrollment
Guarantor: RNDr. Zdeněk Lánský, Ph.D.
Mgr. Aleš Benda, Ph.D.
Teacher(s): Mgr. Aleš Benda, Ph.D.
Marcus Braun, Ph.D.
RNDr. Zdeněk Lánský, Ph.D.
Ing. Dalibor Pánek, Ph.D.
Ing. Petra Prokšová, Ph.D.
Annotation -
Last update: Mgr. Aleš Benda, Ph.D. (15.10.2018)
Recording a single molecular motor protein walking along a microtubule or following diffusion of proteins in living cells became almost routine tasks, allowing to get insights into molecular interactions of biomolecules. Single molecule microscopy and manipulation practical course focuses on optical based methods of detection, characterization and even manipulation of single molecules. The aim is to highlight the uniqueness of single molecule based methods for uncovering the mechanisms of molecular interactions in biophysics, but also to show participants the real laboratory world of single molecule experiments.
The course covers traditional methods of single molecule observation using TIRF based wide-field microscopy and confocal microscopy combined with spectral and excited state lifetime resolved detection. The single molecule manipulation, including force measurement and confocal imaging of the manipulated molecules, is performed using the optical tweezers. The methodologies covered include single particle tracking and various modifications of fluorescence correlation spectroscopy.
Literature -
Last update: RNDr. Nataša Šebková, Ph.D. (06.06.2022)

Single-Molecule Techniques: A Laboratory Manual, Cold Spring Harbor Laboratory Press 2008, ISBN  978-087969775-4

Handbook of Single-Molecule Biophysics, Springer New York 2009, ISBN 978-1-4939-3919-0

Requirements to the exam -
Last update: Mgr. Aleš Benda, Ph.D. (07.10.2019)

Active participation at the course (max. 1 day of absence - excluding Monday) plus short 3 min presentation of your own work or future plans.

Syllabus -
Last update: Mgr. Marie Olšinová, Ph.D. (09.10.2021)

Entry requirements -
Last update: Mgr. Aleš Benda, Ph.D. (21.09.2017)

The course is intended for magister and doctoral students.

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