RNA structure and function - MB150P91E
Title: RNA structure and function
Czech title: Struktura a funkce RNA
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2023
Semester: summer
E-Credits: 3
Examination process: summer s.:oral
Hours per week, examination: summer s.:2/0, Ex [HT]
Capacity: 25
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: English
Additional information: http://www.img.cas.cz/public/skupiny/Stanek.html
Note: enabled for web enrollment
Guarantor: prof. Mgr. David Staněk, Ph.D.
Opinion survey results   Examination dates    E-learning course
Annotation -
The course "RNA Structure and Function" is planned for master and PhD students and extends basic molecular and cell biology courses. The course is intended mainly for students who want to extend their knowledge about regulation of gene expression and about the ever-growing world of RNA. Individual lectures are focused on transcripts of RNA polymerases I, II and II, their synthesis, processing and function. We will also discuss function of non-coding RNAs, riboswitches and ribozymes. The majority of discoveries about RNAs is documented by original scientific publications with a description of key experiments.
Last update: Staněk David, prof. Mgr., Ph.D. (31.05.2019)
Literature -

Genes IX, autor: Benjamin Lewin, (available on-line: http://biology.jbpub.com/book/genes/index.cfm)

Molecular Cell Biology. 5th edition. autor: Lodis, et al. New York: W. H. Freeman; 2003. ISBN-10 0-71674366-3

RNA World 3rd edition, CSHL Press, ISBN 978-087969739-6

Presentations, slides and notes can be also found at https://dl2.cuni.cz/course/view.php?id=326

Last update: Staněk David, prof. Mgr., Ph.D. (05.04.2022)
Requirements to the exam -

Requirements for the exam

The oral exam covers 3-4 topics presented during the course. At least three days before the exam, students must submit a short (1-2 page) essay or review on a topic in RNA biology of their choice. This may also be a summary of a recent paper focusing on RNA. Students may choose the language in which the essay will be written (English or Czech), and the same language will be used in the oral exam.

Last update: Staněk David, prof. Mgr., Ph.D. (05.04.2022)
Syllabus -

Syllabus "Structure and function of RNA"

Introduction

RNA polymerase I transcripts

Synthesis and processing of ribosomal RNA, nuclear structure and function, structure and function of ribosomes


RNA polymerase II transcripts

- regulation of transcription by non-coding RNAs, links between transcription and processing

- long non-coding RNAs, their transcription, processing and function of specific lncRNAs (XIST, HOTAIR, AIR, MALAT1, etc.)

- short non-coding RNAs, snRNA and snoRNA biogenesis, scaRNA. telomerase structure and function

- pre-mRNA processing (processing at 5' and 3' ends, editing, polyA), mRNA localization and stability

- RNA splicing

- regulation of alternative splicing

- cellular structures involved in RNA metabolism


RNA polymerase III transcripts

Transcription, processing and function of tRNA, 7SK, SRP, U6, RNA P and MRP


Catalytic RNAs

ribozymes and ribosomes, spliceosome and ribosome as ribozymes


RNAi

Synthesis, processing and function of miRNA, piwiRNA, siRNA

Translated with www.DeepL.com/Translator (free version)

Last update: Staněk David, prof. Mgr., Ph.D. (05.04.2022)