RNA structure and function - MB150P91E

• Title: RNA structure and function
• Czech title: Struktura a funkce RNA
• Guaranteed by: Department of Cell Biology (31-151)
• Faculty: Faculty of Science
• Actual: from 2014 to 2017
• Semester: summer
• E-Credits: 3
• Examination process: summer s.:oral
• Hours per week, examination: summer s.:2/0, Ex [HT]
• Capacity: 25
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Additional information: http://www.img.cas.cz/public/skupiny/Stanek.html
• Note: enabled for web enrollment
• Guarantor: prof. Mgr. David Staněk, Ph.D.
• Teacher(s): prof. Mgr. David Staněk, Ph.D.

Annotation

Last update: prof. Mgr. David Staněk, Ph.D. (31.05.2019)

The course "RNA Structure and Function" is planned for master and PhD students and extends basic molecular and cell biology courses. The course is intended mainly for students who want to extend their knowledge about regulation of gene expression and about the ever-growing world of RNA. Individual lectures are focused on processing of mRNA as well as synthesis, processing and function of non-coding RNAs. The majority of discoveries about RNAs is documented by original scientific publications with explanation of key experiments.

Literature

Last update: prof. Mgr. David Staněk, Ph.D. (31.05.2019)

enes VIII, autor: Benjamin Lewin, Publisher: Prentice Hall | ISBN: 0131238264

Molecular Cell Biology. 4th edition. autor: Lodish H, Berk A, Zipursky SL, et al. New York: W. H. Freeman; 2000. ISBN-10: 0-7167-3136-3 (free available on http://www.ncbi.nlm.nih.gov/books/NBK21475/)

RNA World 3rd edition, CSHL Press, ISBN 978-087969739-6

Requirements to the exam

Last update: RNDr. Nataša Šebková, Ph.D. (20.02.2018)

Requirements for the exam

3-4 topics presented during the course are discussed during the oral exam. At least three days before the exam students have to submit a short (1-2 pages) assay or review about a topic of their choice from RNA biology. The students can choose the language of the assay (English or Czech) and the same language will be used during the oral exam.

Syllabus

Last update: RNDr. Nataša Šebková, Ph.D. (20.02.2018)

Sylabus "RNA structure and function"

Introduction

RNA polymerase I transcripts

• ribosomal RNA synthesis and processing, nucleolar structure and function, ribosome structure, and function

RNA polymerase II transcripts

• regulation of transcription through RNA network, connection between transcription and processing

• snRNA and snoRNA biogenesis, scaRNAs. telomerase structure and function

• pre-mRNA processing (capping, splicing, editing, polyA) , alternative splicing, mRNA localization and stability

• cellular structures involved in RNA metabolism

RNA polymerase III transcripts

• transcription, processing and function of tRNAs, 7SK, SRP, U6, RNA P and MRP

Catalytic RNAs

• ribozymes and riboswitches, spliceosome and ribosome as ribozymes

Long non-coding RNAs

• transcription, processing andfunction of HOTAIR, Air, MALAT1 and 2

RNAi

• synthesis, processing and function of miRNA, piwiRNA, siRNA