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This course will address fundamental questions about the evolution, function and importance of innate immune system. We will examine how this evolutionary older part of immune system distinguishes between self and foreign substances, discuss molecules that play an essential role in recognition and removal of microbial invaders as well as describe multiple signaling pathways regulating innate immune responses. Course will characterize in detail the invertebrate innate immune system exemplified in the model organism of Drosophila melanogaster. Innate immune responses in mammals as well as their functional links to adaptive immune system will be explored. Further, the two branches of the innate immune system, humoral factors and cells, will be discussed. Finally, we will cover abnormal innate immune responses and how they lead to illness. The main course objective is to understand how the elements of innate immune system work together to achieve health and immune homeostasis. In addition, students will learn to critically read and discuss scientific papers and to evaluate data and methodologies in this quickly progressing field of immunology.
Last update: Půta František, doc. RNDr., CSc. (18.03.2019)
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Course description. This course will address fundamental questions about the evolution, function and importance of innate immune system. We will examine how this evolutionary older part of immune system distinguishes between self and foreign substances, discuss molecules that play an essential role in recognition and removal of microbial invaders as well as describe multiple signaling pathways regulating innate immune responses. Course will characterize in detail the invertebrate innate immune system exemplified in the model organism of Drosophila melanogaster. Innate immune responses in mammals as well as their functional links to adaptive immune system will be explored. Further, the two branches of the innate immune system, humoral factors and cells, will be discussed. Finally, we will cover abnormal innate immune responses and how they lead to illness. The main course objective is to understand how the elements of innate immune system work together to achieve health and immune homeostasis. In addition, students will learn to critically read and discuss scientific papers and to evaluate data and methodologies in this quickly progressing field of immunology. Last update: Půta František, doc. RNDr., CSc. (15.04.2009)
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Recent relevant articles in Nature Reviews in Immunology, Immunity, Nature, Science Roitt's Essential Immunology, Peter J. Delves, Seamus J. Martin, Dennis R. Burton and Ivan M. Roitt Last update: Brdičková Naděžda, Mgr. Bc., Ph.D. (05.04.2012)
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Exam. The grade for the course will be based on one final take-home exam. There will be four questions for the final exam (maximum one page per answer). Exams will be handed out at the end of the final class and will be due on the third day, at no later than 5:00pm. The time limit will be very strict. A penalty of 20 pts/100 pts per day will be docked for late exams. Answers for the take-home exams should be done independently. Last update: Půta František, doc. RNDr., CSc. (21.10.2008)
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1. Intro & general topics, innate immunity--historic/chronological overview; basic groups of microbes, virulence factors.
Modul I: Innate immunity in invertebrates
2. Innate immunity, concept and general characteristics, comparison with adaptive immunity, self-non-self recognition, Danger theory versus infectious-non-self theory. Invertebrate models of innate immunity.
3. Drosophila as a model, multiple levels of defense. Antimicrobial peptides: properties, classification, structure, mode of action.
4. Discovery and function of Drosophila Toll receptors in development and immunity; Rel transcription factors, microbial recognition systems; Toll and imd signaling pathways.
5. Detection of viruses in drosophila, receptors and signaling pathways. Unusual innate immune molecules of invertebrates. Drosophila genome and innate immunity. Future for invertebrate innate immune system research.
6. Tutorial
Modul II: Innate Immunity in mammals
7. Parallels with invertebrate system, the co-evolution of innate and adaptive system, complement systems, cells involved in body defense: leukocytes, macrophages, neutrophils, dentritic cells, mast cells. Overview of phagocytic defense, Oxidative burst Inflammation.
8. Molecular mechanism of innate immune responses: Sepsis model, discovery of Toll-like receptors, adaptor molecules, signaling pathways.
9. Intracellular NOD and NOD-like receptors and their signaling pathways; C-type lectin receptors and their signaling pathways; Viral detection by innate immune system: RIG and RIG-like receptors and their signaling pathways.
10. Links between innate and adaptive immunity: Dentritic cells as a cellular link, types of DC receptors, cytokine secretion profiles; B-1 cells and natural IgM antibody, gamma/delta T cells, NK and NKT cells.
11. Innate immune system in health and disease.
12. Tutorial and final exam questions.
Last update: Půta František, doc. RNDr., CSc. (21.10.2008)
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Prerequisite. While a prerequisite for this course is a basic background in Immunology obtained from recent undergraduate courses, it is not absolutely required. Students who are missing background knowledge in some areas should fill the gaps from the textbook, published reviews, discussions with colleagues, or advice from faculty members.
Last update: Půta František, doc. RNDr., CSc. (21.10.2008)
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