Structure and Function of Cytoskeleton - MB150P67

• Title: Struktura a funkce cytoskeletu
• Czech title: Struktura a funkce cytoskeletu
• Form of teaching: lecture
• Guaranteed by: Department of Cell Biology (31-151)
• Faculty: Faculty of Science
• Actual: from 2022 to 2025
• Duration in semesters: 1
• Semester: winter
• E-Credits: 3
• Examination process: winter s.:
• Hours per week, examination: winter s.:2/0, Ex [HT]
• Capacity: 30
• Maximum number of enrolled students: 30
• Min. number of students: unlimited
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: Czech
• Explanation: prednaska se kona na UMG AVCR, vzdy v pondeli 15:30, poslucharna 3 patro
• Additional information: http://www.img.cas.cz/dbc/
• Note: enabled for web enrollment
• Guarantor: doc. RNDr. Pavel Dráber
• Teacher(s): doc. RNDr. Pavel Dráber

Annotation

English

Please note, the lectures are given in czech language only.

Following topics are included in the lecture-course Cytoskeleton and membrane skeleton: organisation and function of cytoskeleton, structure and dynamics of microtubules, microtubule-associated proteins, microtubule motor proteins, microtubules and cell division, structure and dynamics of microfilaments, microfilament-associated proteins, microfilament motor proteins, microfilaments and muscle contraction, structure and dynamics of intermediate filaments, proteins associated with intermediate filaments, membrane skeleton, nuclear skeleton, interactions between cytoskeleton subsystems, cytoskeleton and signal transduction, pathological changes of cytoskeleton, methods for study structure and dynamics of cytoskeletal components. This lecture-course concentrates on animal cell models. In the course are presented experimental results from Laboratory of Biology of Cytoskeleton, Institute of Molecular Genetics, Czech Academy of Sciences.

Last update: Libusová Lenka, RNDr., Ph.D. (29.07.2026)

Czech

V přednáškovém cyklu "Struktura a funkce cytoskeletu" je cílem uvést studenty do současné problematiky cytoskeletu živočišných buněk. Budou popsány stavební proteiny cytoskeletálních struktur, jejich uspořádání do polymerů a dalších strukturních celků. Budou vysvětleny mechanismy regulující dynamiku cytoskeletálních struktur a jejich interakce s dalšími komponentami buněk. Dále bude probrána stavba a funkce molekulárních buněčných motorů interagujících s cytoskeletem. Bude vysvětlen význam cytoskeletu pro jednotlivé fáze buněčného cyklu, při přenosu vnitrobuněčných signálů a za patologických stavů buňky.
Při přednášce jsou demonstrovány výsledky experimentů z Oddělení biologie cytoskeletu ÚMG AVČR

Last update: Libusová Lenka, RNDr., Ph.D. (29.07.2026)

Literature

Molecular biology of the cell, Alberts et al., 6^th edition, Garland Science 2015

Chapter 9:            Visualizing cells

Chapter 16:          The cytoskeleton

Chapter 18:          Cell cycle (mitosis)

Chapter 19:          Cell junctions and the extracellular matrix (cell-cell junctions, cell-matrix-junctions)

Last update: Libusová Lenka, RNDr., Ph.D. (28.07.2026)

Course assessment methods and requirements for successful completion, grading scheme

Ústní zkouška z celého rozsahu přednášené látky.

Last update: Libusová Lenka, RNDr., Ph.D. (28.07.2026)

Learning outcomes

Learning Outcomes (Cytoskeleton – Introduction)

After completing the course, the student will be able to:

1. Define the main components of the cytoskeleton (microtubules, microfilaments, intermediate filaments) and their principal building proteins. (Remembering)
2. Describe the structure, composition, and basic properties of microtubules, microfilaments, and intermediate filaments. (Understanding)
3. Explain the principles of nucleation and dynamic instability of microtubules as well as the polymerization dynamics of microfilaments. (Understanding)
4. Differentiate among cytoskeletal proteins based on their functions and interactions with associated proteins. (Understanding / Analyzing)
5. Apply knowledge of cytoskeletal structures to interpret microscopic or biochemical data. (Applying)
6. Analyze the interactions between microtubules, microfilaments, and intermediate filaments within the cellular architecture. (Analyzing)
7. Compare the functions of molecular motors (kinesins, dyneins, myosins) and their roles in intracellular transport. (Analyzing)
8. Evaluate the role of the cytoskeleton in cell signaling, including its connections with the membrane and nuclear skeletons. (Evaluating)
9. Interpret the significance of septins and their specific functions in cell division and cytokinesis. (Understanding / Analyzing)
10. Compare and discuss the organization of cytoskeletal systems in eukaryotes and prokaryotes, emphasizing evolutionary relationships. (Analyzing / Evaluating)
11. Explain how defects in cytoskeletal components contribute to pathological conditions such as neurodegenerative diseases and cancer. (Understanding / Applying)
12. Design appropriate experimental approaches and techniques for studying the cytoskeleton (e.g., fluorescence microscopy, FRAP, cryo-EM). (Creating / Applying)

Last update: Libusová Lenka, RNDr., Ph.D. (29.07.2026)

Course registration requirements

Zvládnout výuku v anglickém jazyce. Zkouška je pro česky mluvící studenty vedena v češtině.

Last update: Libusová Lenka, RNDr., Ph.D. (28.07.2026)