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Course, academic year 2025/2026
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Cells and Tissues in vitro - MB150P23
Title: Buňky a tkáně in vitro
Czech title: Buňky a tkáně in vitro
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2022
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:1/2, C+Ex [HT]
Capacity: 80
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Explanation: Praktická cvičení ZÁPIS - MOODLE - turnusy po 10 studentech.
Additional information: https://teams.microsoft.com/l/team/19%3a5967351dce504d53b6b018271040e571%40thread.tacv2/conversations?groupId=df609ae4-b9c2-4228-811d-311a83d2e1f0&tenantId=bece27e5-d902-4c70-88c0-661be1b17dd1
Note: enabled for web enrollment
Guarantor: RNDr. Tereza Tlapáková, Ph.D.
Teacher(s): RNDr. Tereza Tlapáková, Ph.D.
Annotation -
During the anti-epidemic measures, the lectures take place online according to the schedule through the Microsoft Teams communication platform.
Practice takes place in person in shifts of 10 students.


Theory and practice of cell and tissue culture. 1/3 of lectures, 2/3 practical class. Materials, sterilization, aseptic technique, media, supplements, disagregation, maintenance of lines, preservation, transformation, cell separation, measurement and quantitation, differentiation, culture of specific cells, gene transfer, karyology.
Last update: Tlapáková Tereza, RNDr., Ph.D. (07.10.2020)
Literature - Czech

Freshney, R. Ian: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 7th Edition. Wiley-Blackwell, 2016 (ISBN: 978-1-118-87365-6).

 

Last update: Šebková Nataša, RNDr., Ph.D. (21.02.2018)
Requirements to the exam -

Credit from practical courses: 100% presence.
Oral exam.

 

The exam requirements at the time of anti-epidemic measures remain (so far) the same.

 

 

Last update: Tlapáková Tereza, RNDr., Ph.D. (07.10.2020)
Syllabus -

Lectures:

1. Equipment of the laboratory: sterile area, laminar flow, incubators. Materials: glass, plastic, metals.

Water: contamination, distillation, reverse osmosis, deionization, carbon filtration, sterilization.

2. Washing up. Sterilization. Aseptic techniques. Laboratory safety and biohazards. Contamination: mycoplasmas etc. The culture environment: osmolality, pH, buffering, temperature, gas phase.

3. Cell and tissue cultures: animal culture, feeder layer. Media and supplements: balanced salt solution, defined media, serum, serum-free media, selection of medium and serum, other supplements.

4. Disaggregation of the tissue and primary culture: isolation of the tissue, enzymatic disaggregation, collagenase, filtration, centrifugation. Maintenance of the culture: replacement of medium, cell lines, cloning, cell freezing and thawing.

5. Cell counting: hemocytometer, cell counter, calibration. Methods of cell separation: affinity methods, flow cytophotometry. Histochemistry, quantitation of DNA and proteins.

6. Karyology: chromosome preparation, chromosome banding and painting, hybridization.

7. Cell transformation: immortalization, contact inhibition, cell fusion, gene transfer.

Practical class:

1. Primary culture, preparation of medium.

2. Routine maintenance: replacement of medium, cell freezing and thawing.

3. Cell cloning in the presence of a feeder layer.

4. Chromosome preparation and banding.

5. Immunofluorescence.

The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362

Last update: Rubešová Jana, RNDr., Ph.D. (14.09.2019)
Learning outcomes -

1. Remember

  • Lists the basic types of animal cell and tissue cultures and their applications without errors in a written test.
  • Identifies key components of laboratory equipment (laminar flow hood, incubator, centrifuge, microscope) and explains their functions.
  • Describes basic principles of sterilization, types of media, and commonly used supplements.
    Assessment method: Oral examination
    Time frame: By the end of the semester

2. Understand

  • Explains the importance of sterile conditions when working with cell cultures and justifies the choice of specific sterilization techniques.
  • Interprets the composition of culture media and explains how individual components affect cell growth.
  • Summarizes the principles of cell dissociation, cell population separation, basic histochemical and karyological techniques, cell transformation, and targeted modulation of gene expression in cultured cells.
    Assessment method: Discussion

3. Apply

  • Independently prepares the workspace in accordance with biosafety regulations.
  • Establishes and maintains a cell culture following a standard laboratory protocol.
  • Carries out basic laboratory techniques (cell disaggregation, cell counting, protein and DNA determination) without critical errors.
  • Applies correct procedures when handling cell cultures during practical sessions.
    Assessment method: Observation during practical exercises
    Relevance: Direct link to laboratory practice

4. Analyze

  • Distinguishes appropriate cell culture methods depending on the type of cell line.
  • Analyzes the results of cell observation (morphology, growth, viability) and interprets the obtained data.
  • Identifies potential sources of contamination or culture failure and proposes solutions.

5. Evaluate

  • Assesses the quality of a cell culture based on experimental data.
  • Critically evaluates the suitability of applied methods (e.g., transfection, immortalization) with respect to the experimental objective.
  • Observes and defends safety rules and ethical principles in working with cell cultures.
    Assessment method: Oral examination

6. Create

  • Designs a simple experiment using cell cultures (including method and material selection).
  • Prepares a structured laboratory protocol in accordance with good laboratory practice.
  • Formulates experimental conclusions based on obtained results.

 Summary of Graduate Competences: The graduate of this course is able to work safely, independently, and critically with animal cell and tissue cultures. They are familiar with laboratory equipment, apply standard in vitro methods, and are prepared for subsequent experimental and research-oriented courses.

 

Last update: Tlapáková Tereza, RNDr., Ph.D. (29.01.2026)
 
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