SubjectsSubjects(version: 901)
Course, academic year 2022/2023
  
Cells and Tissues in vitro - MB150P23
Title: Buňky a tkáně in vitro
Czech title: Buňky a tkáně in vitro
Guaranteed by: Department of Cell Biology (31-151)
Faculty: Faculty of Science
Actual: from 2021
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:1/2 C+Ex [hours/week]
Capacity: 80
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Explanation: Praktická cvičení probíhají prezenčně v turnusech po 10 studentech.
Additional information: https://teams.microsoft.com/l/team/19%3a5967351dce504d53b6b018271040e571%40thread.tacv2/conversations?groupId=df609ae4-b9c2-4228-811d-311a83d2e1f0&tenantId=bece27e5-d902-4c70-88c0-661be1b17dd1
Note: enabled for web enrollment
Guarantor: RNDr. Tereza Tlapáková, Ph.D.
Teacher(s): RNDr. Tereza Tlapáková, Ph.D.
Opinion survey results   Examination dates   Schedule   
Annotation -
Last update: RNDr. Tereza Tlapáková, Ph.D. (07.10.2020)
During the anti-epidemic measures, the lectures take place online according to the schedule through the Microsoft Teams communication platform.
Practice takes place in person in shifts of 10 students.


Theory and practice of cell and tissue culture. 1/3 of lectures, 2/3 practical class. Materials, sterilization, aseptic technique, media, supplements, disagregation, maintenance of lines, preservation, transformation, cell separation, measurement and quantitation, differentiation, culture of specific cells, gene transfer, karyology.
Literature - Czech
Last update: RNDr. Nataša Šebková, Ph.D. (21.02.2018)

Freshney, R. Ian: Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, 7th Edition. Wiley-Blackwell, 2016 (ISBN: 978-1-118-87365-6).

 

Requirements to the exam -
Last update: RNDr. Tereza Tlapáková, Ph.D. (07.10.2020)

Credit from practical courses: 100% presence.
Oral exam.

 

The exam requirements at the time of anti-epidemic measures remain (so far) the same.

 

 

Syllabus -
Last update: RNDr. Jana Rubešová, Ph.D. (14.09.2019)

Lectures:

1. Equipment of the laboratory: sterile area, laminar flow, incubators. Materials: glass, plastic, metals.

Water: contamination, distillation, reverse osmosis, deionization, carbon filtration, sterilization.

2. Washing up. Sterilization. Aseptic techniques. Laboratory safety and biohazards. Contamination: mycoplasmas etc. The culture environment: osmolality, pH, buffering, temperature, gas phase.

3. Cell and tissue cultures: animal culture, feeder layer. Media and supplements: balanced salt solution, defined media, serum, serum-free media, selection of medium and serum, other supplements.

4. Disaggregation of the tissue and primary culture: isolation of the tissue, enzymatic disaggregation, collagenase, filtration, centrifugation. Maintenance of the culture: replacement of medium, cell lines, cloning, cell freezing and thawing.

5. Cell counting: hemocytometer, cell counter, calibration. Methods of cell separation: affinity methods, flow cytophotometry. Histochemistry, quantitation of DNA and proteins.

6. Karyology: chromosome preparation, chromosome banding and painting, hybridization.

7. Cell transformation: immortalization, contact inhibition, cell fusion, gene transfer.

Practical class:

1. Primary culture, preparation of medium.

2. Routine maintenance: replacement of medium, cell freezing and thawing.

3. Cell cloning in the presence of a feeder layer.

4. Chromosome preparation and banding.

5. Immunofluorescence.

The course is taught with the support of the project reg. number CZ.02.2.69/0.0/0.0/16_015/0002362

 
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