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Viral Biotechnology - MB140C67

Title:	Viral Biotechnology
Czech title:	Virové biotechnologie
Form of teaching:	practical classes
Guaranteed by:	Department of Genetics and Microbiology (31-140)
Faculty:	Faculty of Science
Actual:	from 2023
Duration in semesters:	1
Semester:	summer
E-Credits:	3
Examination process:	summer s.:
Hours per week, examination:	summer s.:0/1, C [TS]
Capacity:	12
Maximum number of enrolled students:	12
Min. number of students:	6
4EU+:	no
Virtual mobility / capacity:	no
State of the course:	taught
Language:	English
Level:	specialized
Note:	enabled for web enrollment

Guarantor:	RNDr. Lenka Horníková, Ph.D.
Teacher(s):	Mgr. Martin Fraiberk, Ph.D. RNDr. Lenka Horníková, Ph.D. RNDr. Hana Španielová, Ph.D.
Class:	Ultracentrifuga a 3 rotory Gradientový PCR cycler Původní předmět

Opinion survey results SS schedule

Annotation -

One-week practical course is designed to introduce students to the methods of viral biotechnology. Students gain
strong theoretical background about the basic principles of viral expression systems, their possibility of usage for
recombinant proteins expression or for production of virus-like particles and methods of their purification. Using
virus-like particles as a model, the students gain practical experience with the methods of production (work with
cell cultures and recombinant viruses) and purification (centrifugation in density gradients) of virus like particles.

Last update: Lichá Irena, RNDr., CSc. (11.05.2021)

Course assessment methods and requirements for successful completion, grading scheme -

For passing the course, the following requirements must be fulfilled:

Participate actively in the course; any unexcused absence may be reason for disqualification from the course.
During the course, focus only on activities related to the course.
Submit protocols and answers to control questions due to date specified. This text can be returned for revision only once after consultation, credit will not be granted in case of repeated deficiencies.

Last update: Horníková Lenka, RNDr., Ph.D. (17.02.2022)

Syllabus -

Students will be diveded into groups (2 students per group) and each group will isolate VLPs by three types of centrifugation - differencial and zonal centrifugation, isopycnic centrifugation. Purity of the VLPs will be characterized using electron microscopy technique.

Last update: Horníková Lenka, RNDr., Ph.D. (07.04.2026)

Learning outcomes -

After completing the course, the student will:

• Describe the basic principle of viral expression system (with emphasis on the baculovirus expression system) and discuss their usage in producing of VLPs.

• Explain the principle of different centrifugation methods and compare their advantages and disadvantages in context of VLPs isolation.

• Independently perform VLPs isolation, purification and characterization by electron microscopy.

• Be able to analyse and interpret experimental results, including the evaluation of electrophoretic records, and clearly process them in the form of a laboratory protocol.

• Discuss the most reliable centrifugation method for VLPs isolation according to data obtained during the course.

• Design own protocol for isolating VLPs.

Last update: Horníková Lenka, RNDr., Ph.D. (13.01.2026)

Course registration requirements

· The course starts on Monday, 23.3.2026 (preliminary date) at 9.00 am in BIOCEV building (Prumyslova 595; Vestec), laboratory L1-012. Meeting point is at reception at 9:00. Enrolled participants are allowed to enter the laboratory only in laboratory clothing and shoes.

· Each participant must be familiar with the instruction manual for the course, which is published at https://dl2.cuni.cz/course/view.php?id=4575 within the course "Viral Biotechnology". Enrollment key: will be provided to participants by e-mail (after 27.2.2026)

Last update: Horníková Lenka, RNDr., Ph.D. (13.01.2026)

The course does not include work placement