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Course, academic year 2023/2024
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Practical course from cytogenetics - MB140C05
Title: Praktická cvičení z cytogenetiky
Czech title: Praktická cvičení z cytogenetiky
Guaranteed by: Department of Genetics and Microbiology (31-140)
Faculty: Faculty of Science
Actual: from 2020
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/1, C [TS]
Capacity: 20
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Level: specialized
Explanation: jednotýdenní turnusové praktikum
Note: enabled for web enrollment
Guarantor: doc. RNDr. Jiří Král, CSc.
Teacher(s): Mgr. Martin Forman
doc. RNDr. Jiří Král, CSc.
Annotation -
Practical course from cytogenetics is focused on methods of basic cytogenetics, namely production of chromosome preparations from plant and animal tissues, banding techniques, and detection of nucleolus organizer regions by silver staining. Obtained mitotic and meiotic preparations are evaluated. Associated excursion into laboratory of human cytogenetics is focused on techniques of molecular cytogenetics as well as computer analysis of chromosome images.
Last update: SPIDER (19.04.2007)
Literature -

Appels R., Morris B.S. Gill, May C.E. (1998). Chromosome biology. Kluwer Academic Publishers.

Clark M.S., Wall W.J. (1996). Chromosomes, the complex code. Chapman x Hall. London.

John B. (1990). Meiosis. Cambridge University Press. Cambridge.

Macgregor H.C. (1993). An introduction to animal cytogenetics. Chapman x Hall. London.

Lacey A.J. (ed.)(1999) Light microscopy in biology: a practical approach. Second edition. Oxford University Press. Oxford.

Popescu P., Hayes H., Dutrillaux B. (eds.) (2000). Techniques in animal cytogenetics. Springer Verlag. Berlin.

Strachan T., Read A.P. (1999). Human molecular genetics 2. John Wiley and Sons Inc. New York.

Sumner A.T. (2003). Chromosomes: organization and function. Blackwell Publishing. Malden x Oxford.

Therman E., Susman M.(1993). Human chromosomes: structure, behavior, and effects. Springer Verlag New York.

Traut W. (1991). Chromosomen. Klassische und molekulare Cytogenetik. Springer Verlag. Berlin.

Verma R.S. (1990). The Genome. VCH Publishers, Inc. New York.

Last update: SPIDER (19.04.2007)
Requirements to the exam -

To get credits, active participation in the practicle course is required.

Last update: Schierová Michaela, RNDr., Ph.D. (24.10.2019)
Syllabus -

I. Production of chromosome preparations from plant tissues. Observation of C bands as well as chromosome aberrations using

permanent preparations

II. Excursion into laboratory of human cytogenetics. Presentation of methods of molecular cytogenetics as well as computer

analysis of chromosome images

III. Production of chromosome preparation from somatic and sexual tissues of mouse. Banding of selected preparations

(C and G bands), detection of nucleolar organizer regions by silver nitrate. Observation and evaluation of preparations.

IV. Observation and evaluation of meiotic preparations(protozoans, grasshopers, spiders, mouse, human)

Last update: SPIDER (19.04.2007)
 
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