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Course, academic year 2012/2013
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Plant biotechnology and gene engineering - MB130P19I
Title: Biotechnologie a genové inženýrství rostlin
Guaranteed by: Department of Experimental Plant Biology (31-130)
Faculty: Faculty of Science
Actual: from 2012 to 2013
Semester: winter
E-Credits: 3
Examination process: winter s.:
Hours per week, examination: winter s.:2/0, Ex [HT]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Explanation: Čas a místo konání přednášky bude upřesněno na semináři 2.10. 15 :00, V5 II.p
Additional information: http://kfrserver.natur.cuni.cz/lide/opat/biotech/index.html
Note: enabled for web enrollment
Guarantor: prof. RNDr. Zdeněk Opatrný, CSc.
Teacher(s): doc. RNDr. Jindřich Bříza, CSc.
RNDr. Lukáš Fischer, Ph.D.
prof. RNDr. David Honys, Ph.D.
Ing. Marie Křístková
Miluše Kusendová
prof. doc. Ing. Tomáš Macek, CSc.
prof. RNDr. Zdeněk Opatrný, CSc.
prof. Ing. Petr Smýkal, Ph.D.
Opinion survey results   Examination dates   Schedule   
Annotation -
The lecture gives a survey of plant biotechnologies based on current knowledge in plant cell biology and mechanisms controlling behaviour of cells and cellular complexes (tissues, organs, embryos) both in vivo and in vitro. Further, it deals with interactions between plants and microorganisms as well as cellular and molecular mechanisms of stresses effect on plant organisms. It includes characteristics of cellular division, differentiation and ontogenesis in in vitro plant cultures; programmed cell death and senescence are discussed. The methods, contemporary results, profits and risks of modern molecular approaches, including GMO construction and use are discussed.
Last update: Konrádová Hana, RNDr., Ph.D. (15.03.2019)
Literature - Czech

Základní studijní literatura:

Ondřej M. (1992) Genové inženýrství kulturních rostlin. Praha: Academia. ISBN 80-200-0310-X.

Pavlová L. (2005) Fyziologie rostlin. Skriptum PřF UK v Praze.

Vondrejs V. (2010) Otazníky kolem genového inženýrství. Praha: Academia. ISBN 978-80-200-1892-2.

Doporučená literatura:

Drobník J. (2008) Biotechnologie a společnost. Skriptum PřF UK v Praze. ISBN 978-80-246-1484-7.

Drobník J., Sehnal F. (2009) White Book genetically modified crops. Biology Centre of the Academy of Science of the Czech Republic. ISBN 978-80-86668-05-3.

Halford N. G. (2006) Plant Biotechnology - Current and Future Applications of Genetically Modified Crops. Chichester: John Willey & Sons Ltd. ISBN 10-0-470-02181-0

Last update: Konrádová Hana, RNDr., Ph.D. (15.03.2019)
Requirements to the exam - Czech

ústní zkouška

Last update: Konrádová Hana, RNDr., Ph.D. (15.03.2019)
Syllabus -

1. Plant biotechnologies: history, principles and use of in vitro techniques and models in experimental plant cell biology and their practical application in agroindustrial sphere

2. Cell cultures: the principles of their initiation, the techniques of the work with plant cell lines and protoplast cultures. Regulatory factors of their growth, division, differentiation. Growth and life cycles, cell senescence and programmed cell death .

3. Polarity and its role in the cell ontogenesis . The role of cytoskeleton and other cell structures in the determination of life events of plant cell lines.

4. Phytohormones and plant cell/tissue cultures. Hormonal metabolism of the cell lines, role of phytohormones / growth regulators in signal pathways in vitro.

5. The mechanisms of regeneration in vivo and in vitro. Principles of micropropagation techniques.

6. Plant embryogenesis I: zygotic and somatic, pollen embryogenesis, gynogenesis.

7. Plant embryogenesis II.: molecular aspects. Practical application of haploid/dihaploid plants in breeding, alternative techniques of their production.

8. Mutagenesis and selection in vitro. Somaclonal and gametoclonal variability. Somatic hybridization.

9. Transgenic cell lines and plants: principles of their construction and use as model materials in plant biology. GMO profits in agroindustry. Potential risks, legislative.

10. Plants and microorganisms: principles and consequences of their interactions. Plant cell/tissue cultures application in their theoretical studies.

11. Plants and abiotic stress. Plant cell/tissue cultures as model experimental systems.

12. Primary and secondary metabolism of plant cell/tissue cultures in connection with their practical application in agrofood, chemical and pharmaceutical industry.

Last update: Konrádová Hana, RNDr., Ph.D. (15.03.2019)
 
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