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This practical course will demonstrate classical as well as state-of-the-art cytogenetic techniques. The accent is put
on cytogenetic methods beneficial to taxonomic, biosystematic and phylogeographic studies. Students will get acquainted with preparation of mitotic and meiotic chromosomes, chromosome counting, the course of male meiosis as well as with principles of light and fluorescence microscopy. Molecular cytogenetic techniques will include DNA probe labelling, fluorescene in situ hybridization (FISH), genomic in situ hybridization (GISH) and chromosome painting. Digital processing of captured chromosome images will be shown. Last update: Kubátová Alena, RNDr., CSc. (12.04.2011)
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Schwarzacher T, Heslop-Harrison P (2000) Practical in situ hybridization. BIOS Scientific Publishers Limited.Oxford. pp.203. Liehr T (ed.) 2009. Fluorescence in situ hybridization (FISH). Springer-Verlag. Heidelberg. Berlin. pp.451. Lysak MA, Mandakova T (2012) Analysis of plant meiotic chromosomes by chromosome painting. In Plant Meiosis: Methods and Protocols. Edited by Pawlowski WP, Grelon M. Humana Press. New York. (pp. = jeste neni publikovano) Last update: Kubátová Alena, RNDr., CSc. (26.04.2012)
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písemný test Last update: Lysák Martin A., doc. Mgr., Ph.D. (11.01.2012)
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Day 1
Day 2
Day 3
Last update: Kubátová Alena, RNDr., CSc. (12.04.2011)
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