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Course, academic year 2023/2024
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Practical course of karyological and palynological methods - MB120C112
Title: Karyologické a palynologické praktikum
Czech title: Karyologické a palynologické praktikum
Guaranteed by: Department of Botany (31-120)
Faculty: Faculty of Science
Actual: from 2021
Semester: winter
E-Credits: 4
Examination process: winter s.:
Hours per week, examination: winter s.:0/1, C [TS]
Capacity: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
State of the course: cancelled
Language: Czech
Explanation: nahrazen MB120C51
Additional information: http://www.ibot.cas.cz/fcm/suda/presentation_honza.html
Note: enabled for web enrollment
the course is taught as cyclical
Guarantor: doc. Clément Lafon Placette, Dr.
Opinion survey results   Examination dates   Schedule   
Annotation -
The lectures can be given in English.
Current approaches and methods in karyology (chromosome counting using squash techniques), cytometry (rapid estimation of nuclear DNA content using flow and image cytometries), and palynology (pollen morphology, determination and documentation).
Last update: Abraham Vojtěch, RNDr., Ph.D. (25.10.2019)
Literature -

Krahulcová (1998): Karyologie cévnatých rostlin při aplikaci metod klasického barvení chromozómů

Halbritter, H., Ulrich, S., Grímsson, F., Weber, M., Zetter, R., Hesse, M., Buchner, R., Svojtka, M., Frosch-Radivo, A., 2018. Illustrated Pollen Terminology. Springer International Publishing, Cham. https://doi.org/10.1007/978-3-319-71365-6
open access book, look inside and look forward to pollen

Punt, W., Hoen, P.P., Blackmore, S., Nilsson, S., Le Thomas, A., 2007. Glossary of pollen and spore terminology. Review of Palaeobotany and Palynology 143, 1–81. https://doi.org/10.1016/j.revpalbo.2006.06.008

Beug, H.-J., 2004. Leitfaden der Pollenbestimmung in Mitteleuropa und angrenzenden Gebiete. Verlag Dr. Friedrich Pfeil, München.
Last update: Abraham Vojtěch, RNDr., Ph.D. (25.10.2019)
Requirements to the exam -

active participation + pollen determination test

Last update: Abraham Vojtěch, RNDr., Ph.D. (14.11.2019)
Syllabus -

- important botany textbooks and journals

- methods in palynology, preparation and evaluation of pollen samples

- methods in karyology, chromosome countings using rapid squash method

- flow cytometry - ploidy level and total DNA content analyses

- image cytometry - estimation of nuclear DNA content in relative and absolute units, size determination

- palynology:

  • terminology of pollen morphology + practical observation in permanent slides
  • pollen determination using dichotomous keys
  • software for routine counting, download here , documentation


The image cytometry was supported by the grant project FRVŠ 912/2004: An innovation of Laboratory of flow and image cytometries for a practical tuition

Last update: Abraham Vojtěch, RNDr., Ph.D. (25.10.2019)
 
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