Phytochemical Methods Laboratory II - GFYT010
Title: Laboratoř z fytochemických metod II.
Guaranteed by: Department of Pharmacognosy and Pharmaceutical Botany (16-16230)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2024
Semester: summer
Points: 0
E-Credits: 4
Examination process: summer s.:
Hours per week, examination: summer s.:0/56, C [HS]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Guarantor: doc. PharmDr. Jakub Chlebek, Ph.D.
SS schedule   
Annotation -
As part of the practical exercises, students will study other methods of preparative chromatography for the isolation of pure compounds from fractions obtained from the previous semester within the Laboratory of Phytochemical Methods I., namely the use of preparative HPLC. An integral part of the isolation of pure compounds is their subsequent identification using tandem methods (GC-MS, HPLC-MS), physical and chiroptical methods. Students will also optimize the possibility of changing the structures of isolated substances to achieve increased solubility, stability, detection and biological activity, or bioavailability. An integral part of the practical exercises is the subsequent in silico evaluation of isolated compounds.
Last update: Rydval Miroslav, Ing. (08.07.2025)
Course completion requirements -

100% attandance in practical classes.

Last update: Rydval Miroslav, Ing. (08.07.2025)
Literature - Czech

Doporučená:

  • OPLETAL, L., DRAŠAR, P.. Fytochemické metody. 1. Izolace obsahových látek (laboratorní technika). Praha: Karolinum, 1994, s. ISBN .

  • Ikan, R.. Natural Products. A Laboratory Guide.. London: Academic Press, 1991, s. ISBN .

  • COLEGATE, M.S., MOLYNEUX, R.J.. Bioactive natural products: detection, isolation, and structural determination.. Boca Raton: CRC Press, Taylor & Francis Group, 2008, s. ISBN .

Last update: Rydval Miroslav, Ing. (08.07.2025)
Teaching methods -

Practical classes.

Last update: Rydval Miroslav, Ing. (08.07.2025)
Syllabus -

1. HPTLC-MS analysis of fractions

2. Preparative chromatography of fractions

3. GC, HPLC analysis

4. Physical methods of isolate identification

5. Chiroptical methods of natural substance identification

6. Derivatization of secondary plant metabolites

7. In silico evaluation of achieved results

Last update: Rydval Miroslav, Ing. (08.07.2025)
Learning outcomes -

Prerequisite: theoretical knowledge of the subject Phytochemical Methods (GAPL002). Knowledge of analytical chemistry, organic chemistry.

The subject Laboratory of Phytochemical Methods II. builds on the knowledge and skills acquired in the subjects Phytochemical Methods, Laboratory of Phytochemical Methods I., Chemistry of Natural Substances I., Chemistry of Natural Substances II.
The graduate of the practical exercises is familiar with the use of preparative HPLC for the isolation of pure substances from mixtures/fractions obtained by Flash chromatography, or by other separation techniques. The student has practical skills in the identification of isolated compounds using tandem methods (GC-MS, HPLC-MS), physical and chiroptical methods. Students can modify the structure of isolated substances to achieve increased solubility, stability, detection and biological activity, or bioavailability. The graduate of the practical exercises can evaluate isolated compounds using in silico methods (work with databases, software).

Learning outcomes:
Based on the acquired knowledge and skills, the student:

  • will design a method for the separation of substances using preparative HPLC, including method development, setting parameters in the software, including evaluation of separation, identification, and purity of the isolated compound
  • will identify isolated compounds using tandem methods (GC-MS, HPLC-MS), physical and chiroptical method.
  • knows the principles of preparing derivatives of isolated compounds to achieve increased solubility, stability, detection and biological activity, or bioavailability
  • evaluate and interpret isolated compounds using in silico methods (work with databases, software)
Last update: Rydval Miroslav, Ing. (08.07.2025)
Entry requirements -

Prerequisite: theoretical knowledge of the subject Phytochemical Methods (GAPL002). Knowledge of analytical chemistry, organic chemistry.

Last update: Rydval Miroslav, Ing. (08.07.2025)