Chemical Laboratory Technique - GF415
Title: Chemická laboratorní technika
Form of teaching: lecture+seminar+practical classes
Guaranteed by: Department of Pharmaceutical Chemistry (16-16320)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2026
Duration in semesters: 1
Semester: summer
Points: 0
E-Credits: 3
Examination process: summer s.:
Hours per week, examination: summer s.:0/52, C [HS]
Capacity: unknown / unknown (unknown)
Maximum number of enrolled students: unlimited
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Repeated enrollment: 1 / 1 / - / 1
Guarantor: doc. PharmDr. Mgr. Martin Krátký, Ph.D.
Incompatibility : GF369
Interchangeability : GF369
Is incompatible with: GF369
Is pre-requisite for: GF304, GF400
Is interchangeable with: GF369
Annotation -
This course introduces students to the fundamental methods used in chemical laboratories. Students will learn the functions and proper use of basic laboratory equipment, as well as modern procedures in the synthesis—primarily of organic compounds—together with methods of separation, purification, and characterization of substances.
Last update: Krátký Martin, doc. PharmDr. Mgr., Ph.D. (12.09.2025)
Course completion requirements -

Requirements for Course Credit

  • Attendance at the introductory seminar held before the start of practical instruction.

  • Successful completion of a test on the content of the introductory seminar during the first practical session.

  • Active participation in all practical classes.

  • Submission of all products obtained as specified in the assignments.

  • Submission of handwritten lab reports from each practical exercise to the instructor (reports must be approved).

  • Achieving at least 60% of the points on the credit test.

  • Any additional requirements as specified by the instructor at the first practical session.

Last update: Krátký Martin, doc. PharmDr. Mgr., Ph.D. (12.09.2025)
Literature -

Obligatory:

  • Vinšová, Jarmila. Laboratory manual for organic chemistry. Praha: Karolinum, 1995, 120 s. ISBN .

  • Palát, Karel, Macháček, Miloš. Laboratory manual for general and inorganic chemistry : určeno pro posl. farmaceut. fakulty Univ. Karlovy. Praha: Karolinum, 1993, 109 s. ISBN 80-7066-751-6.

Last update: Krátký Martin, doc. PharmDr. Mgr., Ph.D. (12.09.2025)
Syllabus -

Practical Session Content and Required Theoretical Knowledge

  • Laboratory safety

  • First aid in the chemistry lab

  • Laboratory tour – functions and layout of the facilities

  • Laboratory glassware – functions and correct use

  • Basic lab operations – weighing, volumetric measurements, solution preparation

  • Heating – heat sources, baths

  • Mixing reaction mixtures

  • Cooling – types of condensers; composition of cooling mixtures

  • Purification of solids – crystallization (by cooling hot saturated solutions, by changing solubility, by salting out); use of activated charcoal

  • Purification of liquids (distillation) – simple, fractional, steam, azeotropic, with air condenser, vacuum, fractional vacuum

  • Isolation of solids – evaporation (rotary vacuum evaporator), precipitation, filtration (gravity, fluted filter, use of a Büchner funnel, Wilstätter needle); sublimation

  • Extraction – shake-out (liquid–liquid), multiple extraction, maceration, digestion, percolation, perforation

  • Drying – of liquids and solids; lyophilization (freeze-drying)

  • Gas preparation – generation, drying of gases, handling of gases

  • Characterization of solids – melting point; spectroscopic methods; refractive index; optical activity (specific rotation)

  • Thin-layer chromatography (TLC) – principle, importance in organic synthesis, and methodology

Last update: Krátký Martin, doc. PharmDr. Mgr., Ph.D. (12.09.2025)
Learning outcomes -

Chemical Laboratory Techniques – Course Description and Learning Outcomes

Prerequisites / Background
The course builds on knowledge and skills from General & Bioinorganic Chemistry and General Organic Chemistry, especially competence in chemical calculations and understanding of organic and inorganic reactivity.

Course description
Upon completion, students will know and comply with laboratory safety rules, be able to provide first aid for the most common laboratory accidents, perform basic laboratory calculations, work with standard laboratory equipment, carry out fundamental laboratory techniques and simple syntheses, keep proper laboratory records, and appropriately characterize compounds.

Learning outcomes — students will be able to:

  • Know and follow safety regulations in the chemical laboratory.

  • Apply first-aid procedures in the laboratory when needed.

  • Use correct terminology for laboratory equipment.

  • Perform basic lab skills (weighing, volumetric measurement, pipetting, glassware washing, etc.).

  • Prepare solutions to specification, selecting the appropriate formula(e) and calculating required quantities.

  • Understand and perform core laboratory methods: various crystallizations, extractions, distillation, sublimation, decantation, and TLC.

  • Synthesize simple compounds.

  • Characterize both solid and liquid compounds.

  • Maintain complete laboratory notebooks/records.

Last update: Krátký Martin, doc. PharmDr. Mgr., Ph.D. (12.09.2025)
Course registration requirements -

Enrollment Requirement and Student Equipment

Enrollment prerequisite
The course Chemical Laboratory Techniques may be taken only after earning course credit (zápočet) in General and Bioinorganic Chemistry.

Additional personal equipment for practicals (beyond standard items):

  • White cotton lab coat

  • Safety glasses

  • Indoor shoes (lab slippers)

  • Matches or a lighter

Last update: Krátký Martin, doc. PharmDr. Mgr., Ph.D. (12.09.2025)