SubjectsSubjects(version: 941)
Course, academic year 2022/2023
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Microbiology - GF129
Title: Mikrobiologie
Guaranteed by: Department of Biological and Medical Sciences (16-16150)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2019
Semester: summer
Points: 0
E-Credits: 4
Examination process: summer s.:oral
Hours per week, examination: summer s.:28/14, C+Ex [HS]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Virtual mobility / capacity: no
Key competences:  
State of the course: not taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Explanation: (F,1.r.)
Note: deregister from the exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: RNDr. Klára Konečná, Ph.D.
Classification: Health Care > Basic Sciences
Co-requisite : GF283
Is incompatible with: GF366
Is interchangeable with: GF366
Examination dates   Schedule   
Annotation -
Last update: RNDr. Klára Konečná, Ph.D. (19.12.2017)
The subject microbiology belongs to biological disciplines, providing basic knowledge about biology, morphology, metabolism, genetics and pathogenicity of microorganisms. The emphasis is put on fundamental aspects of relationship microorganism-host-environment and antimicrobial agents influence. The aim of education is focused on preparation of students to extend the basic knowledge for next studies and on application knowledge in medical practise.
Course completion requirements -
Last update: RNDr. Klára Konečná, Ph.D. (19.12.2017)

Credit conditions:
100% attendance in laboratory practice (no absence without leave).

Worked out protocol from each lecture with average classicification at worst 2.0 .

Successfully passed credit test. 

Presentation of the assigned topic.

Exam conditions:
Gained credit.

Successfully passed exam test (20 questions + short essay). 

Oral exam.

Literature - Czech
Last update: RNDr. Klára Konečná, Ph.D. (19.12.2017)


  • Buchta, Vladimír, Horáček, Jiří Horák, Vladimír, Jílek, Petr. Základy mikrobiologie a parazitologie pro farmaceuty. Praha: Karolinum, 1998, 192 s. ISBN 80-7184-565-5.
  • Jílek, Petr, Förstl, Miroslav, Buchta, Vladimír, Kubanová, Petra. Úvod do mikrobiologických vyšetřovacích metod ve zdravotnictví. Praha: Karolinum, 2002, 104 s. ISBN 80-246-0459-0.
  • Votava, Miroslav. Lékařská mikrobiologie obecná. Brno: Neptun, 2001, 247 s. ISBN 80-902896-2-2.
  • Goering, Richard V. Dockrell, Hazel M. Zuckerman, Mark A. Chiodini, Peter L.. Lékařská mikrobiologie. Praha: Stanislav Juhaňák - Triton, 2016, 568 s. ISBN 978-80-7387-928-0.


  • Votava, Miroslav. Lékařská mikrobiologie. Brno: Masarykova univerzita, 2000, 309 s. ISBN 80-210-2272-8.
  • Votava, Miroslav. Lékařská mikrobiologie speciální. Brno: Neptun, 2003, 495 s. ISBN 80-902896-6-5.

Syllabus -
Last update: RNDr. Klára Konečná, Ph.D. (19.12.2017)

Introduction to microbiology. Human and microbes.

Host versus pathogen.

General bacteriology, genetics of bacteria.

Medically important cocci.

Sexually transmitted diseases.

General virology – structure and replication of viruses. Classification of viruses.

Mycotic infections.

Resistance to antibiotics.

Parasitic protozoa nad helmints.

Disinfection, preservatives and sterilization in pharmacy.

Pharmaceutical microbiology.

Mycobacteria and other filamenous bacteria.

Practical organization in microbiological laboratory– safety in microbiological laboratory.

Disinfection, sterilisation – disinfection of hands, equipment of microbiological laboratory, biosafety levels in laboratory, types of biological samples for microbiological examinations, specimen collection, transport of clinical specimen, request form, microbiology of environment – smear from subject surfaces, …  

Microscopy – native and stained mounts (principles, pros and cons, usage).

Cultivation – types, conditions, types of cultivation media and their usage.

Laboratory screening of susceptibility to antimicrobial agents – disc diffusion test, E-test, determination of MIC.

G+ and G- cocci (laboratory diagnostics), G- rods (laboratory diagnostics), Enterobacteriaceae, G- non-fermenting rods.

Mycology (laboratory diagnostics), parasitology (laboratory diagnostics).


Learning resources -
Last update: RNDr. Klára Konečná, Ph.D. (10.04.2019)

Teaching methods -
Last update: RNDr. Klára Konečná, Ph.D. (19.12.2017)

lectures, PPT presentations

seminars – discussion on given topic

practical classes – introduction to basic methods used in microbiological laboratories

Requirements to the exam -
Last update: RNDr. Klára Konečná, Ph.D. (19.12.2017)

knowledge of discipline defined in sylabus of the subject

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