SubjectsSubjects(version: 944)
Course, academic year 2023/2024
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Bioanalysis of drugs - GDFA10
Title: Bioanalytické hodnocení léčiv
Guaranteed by: Department of Pharmaceutical Chemistry and Pharmaceutical Analysis (16-16190)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2019
Semester: both
Points: 0
E-Credits: 0
Examination process: oral
Hours per week, examination: 0/0, Ex [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: full-time
Teaching methods: full-time
Note: course is intended for doctoral students only
enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: doc. PharmDr. Petra Štěrbová, Ph.D.
Incompatibility : GDFA08
Interchangeability : GDFA08
Is incompatible with: GDFA08
Is interchangeable with: GDFA08
Annotation -
Last update: doc. PharmDr. Petra Štěrbová, Ph.D. (25.09.2023)
Bioanalysis of Drugs is aimed at introduction of the most important methods and procedures used in analysis of drugs and metabolites in biological materials. The methods are utilized for investigation of pharmacokinetic, therapeutic drug monitoring, toxicological and forensic analysis etc. Both theoretical and practical training are focused on separation and immunochemical methods, issues of matrix effect, validation of bioanalytical methods and sample preparation techniques. The attention is particularly paid on HPLC and UHPLC coupled with mass spectrometry as well as modern trends in drug bioanalysis. The training is realized in cooperation with Department of Clinical Biochemistry and Diagnosis, Faculty Hospital in Hradec Kralove.
Literature - Czech
Last update: doc. PharmDr. Petra Štěrbová, Ph.D. (25.09.2023)


  • Hansen, Steen, Pedersen-Bjergaard, Stig (eds.). Bioanalysis of pharmaceuticals: sample preparation, separation techniques, and mass spectrometry. Chichester: Wiley, 2015, 318 s. ISBN 978-1-118-71682-3.


  • Ivanov, Alexander R. Lazarev, Alexander V. (eds.). Sample preparation in biological mass spectrometry. New York: Springer, 2011, 811 s. ISBN 978-94-007-0758-0.
  • Wenkui Li, Jie Zhang, Francis L.S. Tse. Handbook of LC-MS bioanalysis: best practices, experimental protocols, and regulations . Hooboken, New Jersey: Wiley, 2013, 684 s. ISBN 9781118159248.
  • Boyd, Bob, Basic, Cecilia Bethem, Robert. Trace quantitative analysis by mass spectrometry. Chichester ; Hoboken: John Wiley & Sons, 2008, 709 s. ISBN 978-0-470-05771-1.
  • Hübschmann, Hans-Joachim (ed.). Handbook of GC/MS:fundamentals and applications. Weinheim: John Wiley, 2007, 863 s. ISBN 978-3-527-31427-0.

Requirements to the exam -
Last update: doc. PharmDr. Petra Štěrbová, Ph.D. (13.02.2019)

Examination topics:

  1. Aims, specific features and main areas of drugs analysis in biological materials
  2. The main types of biological samples, their sampling and storage
  3. The most frequent analytical methods in drug bioanalysis
  4. Chromatographic methods in drug bioanalysis
  5. Methods of quantification of drugs in biological materials
  6. Identifications of drugs and metabolites
  7. Spectral methods in drug bioanalysis
  8. Electroanalytical methods in drug bioanalysis (small molecules, proteins and peptides)
  9. Mass spectrometry in drug bioanalysis
  10. Common sample preparation techniques
  11. Trends in preparation of biological samples
  12. Ultrafiltration and other methods of protein removing
  13. Immunoanalytical methods
  14. Development and optimization of a bioanalytical method
  15. Bioanalytical method validation
  16. Chiral separations in drug bioanalysis
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