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Course, academic year 2022/2023
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Analysis of substances of natural origin - GDFA04
Title: Analýza látek přírodního původu
Guaranteed by: Department of Analytical Chemistry (16-16140)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2019
Semester: both
Points: 0
E-Credits: 0
Examination process:
Hours per week, examination: 0/0, Ex [HT]
Capacity: winter:unknown / unknown (unknown)
summer:unknown / unknown (unknown)
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: Czech
Teaching methods: full-time
Level:  
Note: course is intended for doctoral students only
enabled for web enrollment
you can enroll for the course in winter and in summer semester
Guarantor: prof. RNDr. Dalibor Šatínský, Ph.D.
Annotation -
Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (06.03.2019)
The aim of the course is to present to students the current trends in the analysis of substances of natural origin from the point of view of detection and analytical techniques, and especially from the point of view of effective biologically active substances found in plants, fruits and vegetables, which are most often used in the current directions of bio-pharmacy. These include, in particular, antioxidants, phenolic acids, flavonoids, anthocyans, phytosterols, carotenoids, flavonolignans, alkaloids and others. The aim of the subject is also to clarify the principles of extraction of substances from plant materials using modern extraction techniques such as supercritical fluid extraction and their subsequent separation and identification using modern techniques such as ultra-high performance liquid chromatography (UHPLC) and capillary electrophoresis (CE) in conjunction with mass spectrometry (MS). The subject is also an analysis of mycotoxins, and other contaminants in natural plant materials. An integral part is also the analysis of bioactive substances in food supplements based on plant extracts. Individual directions in the analysis of substances of natural origin will focus on the following areas: - Interpretation of analytical data (NMR spectra, mass spectra, IR spectra, Raman spectra) of substances of natural origin; - high resolution HRMS mass spectrometry in the analysis of natural substances and plant extracts, - HRMS in the analysis and identification of toxics causing poisoning; - Use of rapid LC / HRMS methods to diagnose poisoning early; - use of gas chromatography (GC / MS) in plant extracts analysis - use of CE/MS in plant extracts analysis - statistical analysis of data.
Literature - Czech
Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (06.03.2019)

Povinná:

  • Lubomír Opletal. Přírodní látky a jejich biologická aktivita . Praha: Karolinum Praha, 2016, 698 s. ISBN 9788024620848.
  • Opletal, Lubomír. Sekundární metabolity rostlin. Praha: Univerzita Karlova, nakladatelství Karolinum, 2016, 698 s. ISBN 978-80-246-2084-8.
  • Nováková, Lucie, Douša, Michal. Moderní HPLC separace v teorii a praxi. Praha: Europrint, 2013, 299 s. ISBN 978-80-260-4243-3.
  • Vladimir Havlicek, Jaroslav Spizek. Natural Products Analysis: Instrumentation, Methods, and Applications. Amsterodam: Willey, 2014, 624 s. ISBN 978-1-118-46661-2|.

Doporučená:

Volitelná:

  • Flanagan, Robert James (ed.). Fundamentals of analytical toxicology. Chichester, England ; Hoboken, NJ: John Wiley & Sons, 2007, 505 s. ISBN 978-0-470-31935-2.

Requirements to the exam -
Last update: prof. RNDr. Dalibor Šatínský, Ph.D. (06.02.2019)

Analytical methods for analysis of substances of natural origin.

Detection techniques in the analysis of substances of natural origin.

Analyzes of trace concentrations of toxic substances caused poisons.

Modern trends in the preparation of samples from plant extracts.

Combination of SPE HPLC MS and ICP MS to determine trace concentrations of toxic substances.

Use of gas chromatography (GC / MS) in the analysis of plant extracts

Use of CE / MS in the analysis of plant extracts

Interpretation of analytical data

NMR spectra, mass spectra, IR spectra, and Raman spectra of substances of natural origin

 
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