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The aim of the course is to study the method of mass spectrometry both theoretically and practically. This includes the principles of ionization techniques, the basics of instrumentation in mass spectrometry with an emphasis on current developments, especially in the field of ionization techniques under atmospheric pressure and so-called "ambient" ionization techniques, development in the field of analyzers in mass spectrometry, coupling of mass spectrometry with separation techniques including liquid and gas chromatography and also capillary electrophoresis. Practical knowledge includes interpreting spectra and working with different types of MS records.
Last update: Neznámá Renáta (19.08.2019)
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Last update: Neznámá Renáta (19.08.2019)
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1) Definition of basic concepts Mass spectrometry, important historical milestones, basic components of the mass spectrometer, specifics of the laboratory for mass spectrometry, mass spectrum, isotopic representation of elements in nature 2) Ionization techniques in mass spectrometry Theory, History, and Development of Separation Techniques with Mass Spectrometry Types of ionization techniques - soft, hard, further distribution of ionization techniques. EI, CI - detailed principle and possibilities of use. 3) Ionization techniques at atmospheric pressure ESI, APCI, APPI - detailed principle and usage possibilities. 4) Ambient ionization techniques Trends in the development of ionization techniques. DESI, DART, REIMS - detailed principle and possibilities of utilization, imaging, MALDI. 5) Analyzers in mass spectrometry Definition of parameters for mass analyzers - sensitivity, resolution and accuracy of matter. Different types of weight analyzers. Flight analyzers - quadrupole, TOF, magnetic Trap analyzers: ion traps, orbital trap, ICR Tandem mass spectrometry. Hybrid mass analyzers (Q-TOF, Q-IT ...) 6) Types of records in mass spectrometry MS mode: MS scan, SIM, MS / MS mode: SRM, scan of product and precursor ions, scan of neutral losses, separation by ion mobility 7) Combination of mass spectrometry with separation techniques HPLC-MS, SFC-MS, GC-MS, CZE-MS - types of ionization techniques, interface types, practical realization 8) Approaches for quantification of substances using mass spectrometry External and internal standard method for mass spectrometry. Standard addition method. Correct choice of internal standard. Matrix effects. Targeted and non-targeted analysis. 9) Interpretation of mass spectra - hard ionization techniques Rules for interpreting the EI Spectrum. 10) Approaches for identifying substances using mass spectrometry Identification of substances in MS and HRMS. Database, Clarification of Substance Structures. Calibration of masses, measurement of spectra in HRMS. Interpretation of mass spectra - soft ionization techniques 11) Application of mass spectrometry - small molecules Examples of MS utilization in the field of pharmaceutical analysis, bioanalysis, doping control 12) Application of mass spectrometry - proteomics The specifics of MS utilization in protein biomolecules analysis Last update: Neznámá Renáta (19.08.2019)
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