Subjects

Your browser does not support JavaScript, or its support is disabled. Some features may not be available.

Electrophoretic methods in the analysis of biologically active compounds - GDEBM03

Title:	Electrophoretic methods in the analysis of biologically active compounds
Guaranteed by:	Department of Analytical Chemistry (16-16140)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2024
Semester:	both
Points:	0
E-Credits:	0
Examination process:
Hours per week, examination:	0/0, Ex [HT]
Capacity:	winter:unknown / unknown (unknown) summer:unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	English
Teaching methods:	full-time
Level:
Note:	course is intended for doctoral students only enabled for web enrollment you can enroll for the course in winter and in summer semester

Guarantor:	prof. RNDr. Dalibor Šatínský, Ph.D.
Interchangeability :	GDEZCH03
Is interchangeable with:	GDEZCH03

Examination dates WS schedule

Annotation -

Development trends in electrophoretic methods, miniaturization, chip electrophoresis. Electrophoresis in proteomic studies. Non-aqueous capillary electrophoresis and its applications. Selected applications of capillary electrophoresis - separation of peptides and proteins, chiral separation. On-line "stacking" techniques in capillary electrophoresis for the analysis of biological materials. Micellar electrokinetic chromatography. Connection of capillary electrophoresis with advanced detection techniques and mass spectrometry.

Last update: Neznámá Renáta (19.08.2019)

Literature - Czech

Povinná:

Garcia, Carlos D. (ed.). Capillary electrophoresis and microchip capillary electrophoresis principles, applications, and limitations. Hoboken, New Jersey: Wiley, 2013, 394 s. ISBN 978-0-470-57217-7.

Nicola Volpi . Capillary Electrophoresis of Biomolecules . London: Humana Press , 2013, s. ISBN 978-1-62703-295-7.

Doporučená:

Web of Knowledge [online]. Dostupné z: http://apps.webofknowledge.com

sciencediretc [online]. Dostupné z: https://www.sciencedirect.com

Doporučená:

M.G. Khaledi . High Performance Capillary Electrophoresis – Theory, Techniques and Applications, . Amsterdam : Willey, 1998, s. ISBN .

Doporučená:

. . In Electrophoresis, Journal of Separation Sciences, Journal of Chromatography A. (): -.

Last update: Neznámá Renáta (19.08.2019)

Requirements to the exam -

Theoretical aspects of electromigration techniques.

Detection techniques in capillary electrophoresis (CE).

CE in drug and macromolecular analyzes, proteomic analyzes.

Modern trends in electromigration techniques.

The CE for on-line pre-concentration of trace substances.

Trends in micellar electrophoresis and capillary electrochromatography.

Trends in CE coupling with Mass Detection for Biomolecular Analysis.

Last update: Neznámá Renáta (19.08.2019)