SubjectsSubjects(version: 953)
Course, academic year 2023/2024
   Login via CAS
Introduction to Cell Biology - GB273
Title: Úvod do buněčné biologie
Guaranteed by: Department of Biological and Medical Sciences (16-16150)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2021
Semester: winter
Points: 0
E-Credits: 5
Examination process: winter s.:written
Hours per week, examination: winter s.:8/4, C+Ex [HS]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: Czech
Teaching methods: combined
Teaching methods: combined
Level:  
Note: course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: Mgr. Zuzana Svobodová, Ph.D.
Interchangeability : GB258
Is co-requisite for: GB278, GB185, GB169, GB274
Is pre-requisite for: GB281, GB313, GB119, GB191
Annotation -
The main goal of Introduction to Cell Biology is to reveal to the students how the living cell works and teach them how to handle the microscope. Both will give them the background for the subjects in the upcoming years. For that, we introduce them to the structure and function of every single compartment of the cell and its chemical composition. Other areas such as cell communication, cell division, and regulation of the cell cycle are studied. Themes: Structure of prokaryotic and eukaryotic cells, membranes, membrane organelles, DNA and chromosomes, cell communication, cell cycle, reproduction of organisms.
Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
Course completion requirements -

The course consists of lectures and practical classes. The practical classes are completed by getting a credit, which is a necessary condition for access to the final exam test.

 

The credit will be recognize after fulfillment of the following conditions:

1) 100% presence in all practical classes. One PC can be excused in case of serious health or family issues that must be confirmed by a doctor or a lawyer (with regards to the COVID-19 situation).

2) Deliver the Laboratory manual with filled protocols to be checked on time

 

The final exam test is in written form and is composed of 35 questions (theoretical questions approx 30 and approx. 5 questions from practicals).

Types of questions: single best answer, matching, image description.

limit for passing the test: 70% of correct answers

evaluation: excellent: 100 % - 90 %

 very good: 89 % - 80 %

 good: 79 % - 70 %

 

Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
Literature -

Obligatory:

Optional:

  • . . In Nečas, Oldřich. Obecná biologie . Jinočany: H & H, 2000, s. -. ISBN 80-86022-46-3..

Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
Syllabus -

Cell structure

·         definition and general characteristics of cell (cell theory)

·         structure of prokaryotic cell

·         structure of eukaryotic cell (plant and animal)

 

Chemical composition of living systems

·         water and inorganic substances

·         low molecular weight organic molecules: saccharides, organic acids, amino acids, nucleotides, phospholipids

·         high molecular weight organic molecules: polysaccharides, proteins, nucleic acids

 

Nucleic acids and chromosomes I

·         structure of DNA

·         chromosome structure in prokaryotes and viruses

·         chromosome structure in eukaryotes

 

Nucleic acids and chromosomes II

·         replication

·         transcription

·         translation

 

Membranes I

·      membrane structure

o   lipid bilayer

o   molecular structure of membranes – lipids, proteins, saccharides

·      cytoplasmic membrane

 

Membranes II

·           membrane transport

·           carrier proteins

·           ion channels and the electrical properties of membranes

 

Membrane organelles I

·         mitochondria

o   the structure

o   cellular respiration – Krebs cycle, electron-transport chain, oxidative phosphorylation

·         chloroplasts

o   the structure

o   photosynthesis – light reactions and dark reactions

 

Membrane organelles II

·         endoplasmic reticulum

·         Golgi apparatus

·         endosomes

·         lysosomes

·         peroxisomes

·         intracellular vesicular transport

o   transport from the endoplasmic reticulum through the Golgi apparatus

o   transport from the trans Golgi network to lysosomes

o   transport into the cell from the plasma membrane: endocytosis

o   transport from the trans Golgi network to the cell exterior: exocytosis

 

Cell communications

·         general principles of cell communication

·         signal molecules

·         receptors

o   ion-channel–coupled receptors

o   G-protein–coupled receptors

o   enzyme–coupled receptors

 

Cell division

·         cell cycle - four phases (G1, S, G2, M)

·         mitosis - prophase, prometaphase, metaphase, anaphase, telophase, cytokinesis

·         meiosis

 

Cell cycle control system 

·         regulation of the cell cycle

·         cell differentiation

·         cell death

 

Reproduction of organisms

  • asexual reproduction
    • characteristics
    • binary division, vegetative reproduction, amitosis
  • sexual reproduction
    • characteristics
    • gametogenesis - spermatogenesis, oogenesis, fertilization
Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
Learning resources - Czech

https://intranet.faf.cuni.cz/Studijni-materialy/KBLV/

Moodle2: https://dl2.cuni.cz/ : FAF/KBLV/Kurz Buněčné biologie

Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
Teaching methods -

Lectures - theory

Moodle - videos, "quizzes", "questions and answers", make-up own "glossary"

Practical classes - microscopy, observation of plant and animal cells, function of organelles, osmotic effects in plant and animal cells, cell reproduction, tissue cultures

Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
Requirements to the exam -

The form of an exam is a test composed of 35 questions (30 questions from theory and 5 questions from practicals).

Types of questions: single best answer, matching, image description.

  • limit for passing the test: 70% of correct answers

  • evaluation: excellent: 100 % - 90 %

     very good: 89 % - 80 %

     good: 79 % - 70 %

The student should show knowledge across the subject of Cell Biology, specifically in the following areas:

1.      Definition and general characteristics of cell

2.      Structure of prokaryotic cell

3.      Structure of eukaryotic cell 

4.      Cell nucleus

5.      Structure of DNA

6.      Chromosome structure in prokaryotes and viruses

7.      Chromosome structure in eukaryotes

8.      Replication, transcription and translation

9.      Structure of living systems – water, inorganic substances, low molecular weight organic molecules (saccharides, organic acids, amino acids, nucleotides, phospholipids)

10.  Structure of living systems – high molecular weight organic molecules (polysaccharides, proteins, nucleic acids)

11.  Membrane structure

12.  Cytoplasmic membrane

13.  Membrane transport (carrier proteins, ion channels)

14.  Mitochondria (structure, cellular respiration)

15.  Chloroplasts (structure, photosynthesis)

16.  Endoplasmic reticulum

17.  Golgi apparatus

18.  Endosomes

19.  Lysosomes

20.  Peroxisomes

21.  Intracellular vesicular transport

22.  General principles of cell communications

23.  Receptors (basic characteristics, signaling cascade)

24.  Ion-channel–coupled receptors

25.  G-protein–coupled receptors

26.  Enzyme–coupled receptors

27.  Microtubules

28.  Actin filaments

29.  Intermediate filaments

30.  Cell cycle

31.  Mitosis

32.  Meiosis

33.  Cell cycle control system

34.  Cell differentiation

35.  Cell death

36.  Cell stress

37.  Asexual reproduction

38.  Spermatogenesis

39.  Oogenesis

40.  Fertilization

Last update: Svobodová Zuzana, Mgr., Ph.D. (03.10.2023)
 
Charles University | Information system of Charles University | http://www.cuni.cz/UKEN-329.html