SubjectsSubjects(version: 920)
Course, academic year 2022/2023
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Special Instrumental Methods - GB149
Title: Speciální instrumentální metody
Guaranteed by: Department of Analytical Chemistry (16-16140)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2016
Semester: winter
Points: 0
E-Credits: 6
Examination process: winter s.:oral
Hours per week, examination: winter s.:2/3, C+Ex [HT]
Capacity: unknown / unknown (unknown)
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: not taught
Language: Czech
Teaching methods: full-time
Explanation: (ZB, prez.3.r.)
Note: deregister from the exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: prof. RNDr. Dalibor Šatínský, Ph.D.
doc. PharmDr. Hana Sklenářová, Ph.D.
doc. PharmDr. Ludmila Matysová, Ph.D.
Co-requisite : GB094
Interchangeability : GB288
Is interchangeable with: GB288
Examination dates   Schedule   
Annotation -
Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)
Basic course of special instrumental methods. Development, optimization and validation of bioanalytical methods.
Literature -
Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Rücker G., Neugebauer M.,Willems G.G.: Instrumentelle pharmazeutische Analytik, WVG Stuttgart 2001.

Robinson J.W., Skelly Frame E.M., Frame II G.M.. ? Undergraduate Instrumental Analysis, Marcel Dekker, New York, , 2005.

Klouda P.: Moderní analytické metody, nakladatelství Pavel Klouda, Ostrava 2003.

Syllabus -
Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Introduction to special methods of instrumental analysis, classification of methods, principles.

Atomic-absorption spectrophotometry, absorbing medium, resonance spectral lines, instrumentation, analytical applications. Atomic-fluorescence spectrometry.

Luminescence analysis, fluorescence, phosphorescence, chemiluminescence, emission and excitation spectra, fluorimeters, spectrofluorimeters, qualitative and quantitative applications.

Turbidimetry and nephelometry

High-frequency spectroscopic methods, nuclear magnetic resonance, NMR spectrum, analytical applications, mass spectrometry, mass spectrum, analytical applications.

Electromigration methods, electrophoretic mobility, principles of separation, instrumentation, analytical use for the separation of biological macromolecules. Capillary zone electrophoresis, capillary gel electrophoresis, capillary isotachophoresis.

Isoelectric focusation, micellar electrokinetic chromatography. Capillary electrochromatography. Instrumentation, applications.

Ion-exchange chromatography. Ion-exchange phenomena, cation and anion exchangers, ion-exchange equilibria, amino acid analyzers, molecular sieves, gel-permeation chromatography.

Pulse voltammetric methods. Difference pulse polarography. Stripping voltammetry.

Automation of analytical procedures, titrators, types of analyzers and their applications.

Flow-injection analysis, sequential injection analysis, automation of analytical procedures, principles, instrumentation, applications.

Teaching methods -
Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Lectures and practical laboratory training.

Requirements to the exam -
Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Complete practical training and submission of reports from experiments.

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