Subjects

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Special Instrumental Methods - GB149

Title:	Speciální instrumentální metody
Guaranteed by:	Department of Analytical Chemistry (16-16140)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2016
Semester:	winter
Points:	0
E-Credits:	6
Examination process:	winter s.:oral
Hours per week, examination:	winter s.:2/3, C+Ex [HT]
Capacity:	unknown / unknown (unknown)
Min. number of students:	unlimited
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	not taught
Language:	Czech
Teaching methods:	full-time
Level:
Explanation:	(ZB, prez.3.r.)
Note:	deregister from the exam date if a requisite was not fulfilled course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	prof. RNDr. Dalibor Šatínský, Ph.D. doc. PharmDr. Hana Sklenářová, Ph.D. doc. PharmDr. Ludmila Matysová, Ph.D.
Co-requisite :	GB094
Interchangeability :	GB288
Is interchangeable with:	GB288

Examination dates Schedule

Annotation -

Basic course of special instrumental methods. Development, optimization and validation of bioanalytical methods.

Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Literature -

Rücker G., Neugebauer M.,Willems G.G.: Instrumentelle pharmazeutische Analytik, WVG Stuttgart 2001.

Robinson J.W., Skelly Frame E.M., Frame II G.M.. ? Undergraduate Instrumental Analysis, Marcel Dekker, New York, , 2005.

Klouda P.: Moderní analytické metody, nakladatelství Pavel Klouda, Ostrava 2003.

Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Teaching methods -

Lectures and practical laboratory training.

Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Requirements to the exam -

Complete practical training and submission of reports from experiments.

Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)

Syllabus -

Introduction to special methods of instrumental analysis, classification of methods, principles.

Atomic-absorption spectrophotometry, absorbing medium, resonance spectral lines, instrumentation, analytical applications. Atomic-fluorescence spectrometry.

Luminescence analysis, fluorescence, phosphorescence, chemiluminescence, emission and excitation spectra, fluorimeters, spectrofluorimeters, qualitative and quantitative applications.

Turbidimetry and nephelometry

High-frequency spectroscopic methods, nuclear magnetic resonance, NMR spectrum, analytical applications, mass spectrometry, mass spectrum, analytical applications.

Electromigration methods, electrophoretic mobility, principles of separation, instrumentation, analytical use for the separation of biological macromolecules. Capillary zone electrophoresis, capillary gel electrophoresis, capillary isotachophoresis.

Isoelectric focusation, micellar electrokinetic chromatography. Capillary electrochromatography. Instrumentation, applications.

Ion-exchange chromatography. Ion-exchange phenomena, cation and anion exchangers, ion-exchange equilibria, amino acid analyzers, molecular sieves, gel-permeation chromatography.

Pulse voltammetric methods. Difference pulse polarography. Stripping voltammetry.

Automation of analytical procedures, titrators, types of analyzers and their applications.

Flow-injection analysis, sequential injection analysis, automation of analytical procedures, principles, instrumentation, applications.

Last update: POSPISILOVA/FAF.CUNI.CZ (24.06.2008)