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Course, academic year 2022/2023
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Special Methods of Instrumental Analysis (practical classes) - GAF314
Title: Special Methods of Instrumental Analysis (practical classes)
Guaranteed by: Department of Analytical Chemistry (16-16140)
Faculty: Faculty of Pharmacy in Hradec Králové
Actual: from 2022
Semester: winter
Points: 0
E-Credits: 4
Examination process: winter s.:oral
Hours per week, examination: winter s.:0/42, C+Ex [HS]
Capacity: unknown / 60 (unknown)
Min. number of students: 5
Virtual mobility / capacity: no
State of the course: taught
Language: English
Teaching methods: full-time
Level:  
Note: deregister from the exam date if a requisite was not fulfilled
course can be enrolled in outside the study plan
enabled for web enrollment
Guarantor: doc. PharmDr. Hana Sklenářová, Ph.D.
Co-requisite : GAF301, GAF313
Annotation -
Last update: doc. PharmDr. Hana Sklenářová, Ph.D. (16.09.2022)
The aim of the subject is based on practical classes of selected instrumental techniques from the perspectives of practical applications, advantages and also limitations of the use for analysis of different samples. Practical classes are organized in groups of 4 - 5 students. The subject belongs to specialization Pharmaceutical analysis.
Course completion requirements -
Last update: doc. PharmDr. Hana Sklenářová, Ph.D. (16.09.2022)

Requirements for creidt and exam are:

Credit

1.      Participation in all practical classes – (missing practical classes must be revisited or it can be substituted if permitted by the teacher)

2.      Submission of all reports of practical exercises

 

Exam

  1. The exam from Special methods of instrumental analysis is organized in terms presented in the Study information system, the time of exam is always specified.

  2. For the exam, two credits from both lectures and practical classes are needed (Special Methods of Instrumental Analysis – practicals and Special Methods of Instrumental Analysis – lectures).

  3. The exam is oral, exam scope is specified in the Study materials – Study KACH – Pharmacy – Special Methods of Instrumental Analysis - General

Literature -
Last update: doc. PharmDr. Hana Sklenářová, Ph.D. (16.09.2022)

Recommended:

  • Skoog, Douglas A.. Skoog and West's fundamentals of analytical chemistry. [Belmont, Calif.]: Brooks/Cole Cengage Learning, 2013, 958 s. ISBN 978-1-285-05624-1.
  • Robinson, James W., Frame, Eileen M. Skelly Frame, George M.. Undergraduate instrumental analysis. Boca Raton: CRC Press, 2014, 1264 s. ISBN 978-1-4200-635-2.

Syllabus -
Last update: doc. PharmDr. Hana Sklenářová, Ph.D. (16.09.2022)

Syllabus of Special Methods of Instrumental Analysis (practical classes)

  • Potentiometry with ion selective electrodes - determination of low concentration of iodides (impurity) in sample of sodium chloride, measurement selectivity, method of two standard additions and its numeric evaluation.

  • Stripping voltammetry – determination of traces of impurities (heavy metals) – identification and determination in sample of sodium chloride using standard addition method.

  • Capillary zone electrophoresis - determination of sorbic and benzoic acids in unknown sample, calibration.

  • Capillary isotachophoresis – determination of glucosamin in food supplement, comparison with standard solution with known active substance concentration, contactless conductivity detection.

  • UV spectrophotometry – selective determination of constituents of multi-component mixtures (o-hydroxybenzoic and p-hydroxybenzoic acids), selection of optimal wavelengths, evaluation of absorption coefficient, determination of components in unknown sample.

  • High-performance liquid chromatography – identification of components in mixture of active substances (paracetamol, p-hydroxybenzoic acid, acetylsalicylic acid + product of decomposition – salicylic acid), determination of paracetamol, identification of impurity, decomposition of acetylsalicylic acid.

  • Sequential injection analysis – automation of nitrates determination using derivatization reaction in the system of sequential injection analysis, optimization of flow rate, „stop-flow“ technique for spectrophotometric determination, calibration, determination of nitrates in drinking or surface water.

  • Fluorimetry – derivatization of fluorimetric determination of thiamine after oxidation in alkaline conditions, selection of excitation and emission wavelengths, calibration, determination of thiamine in unknown sample.

  • Raman spectrometry – identification of methanol in samples of ethanolic solutions, determination using comparison of band area of methanol and ethanol against low signal in different part of Raman spectrum.

 
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