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Special Methods of Instrumental Analysis (lessons) - GAF313

Title:	Special Methods of Instrumental Analysis (lessons)
Guaranteed by:	Department of Analytical Chemistry (16-16140)
Faculty:	Faculty of Pharmacy in Hradec Králové
Actual:	from 2021
Semester:	winter
Points:	0
E-Credits:	2
Examination process:	winter s.:
Hours per week, examination:	winter s.:28/0, C [HS]
Capacity:	unlimited / unknown (unknown)
Min. number of students:	5
4EU+:	no
Virtual mobility / capacity:	no
Key competences:
State of the course:	taught
Language:	English
Teaching methods:	full-time
Level:
Note:	deregister from the credit exam date if a requisite was not fulfilled course can be enrolled in outside the study plan enabled for web enrollment

Guarantor:	doc. PharmDr. Hana Sklenářová, Ph.D.
Co-requisite :	GAF301
Is co-requisite for:	GAF314
In complex pre-requisite:	GAF1001

Examination dates WS schedule

Annotation -

The aim of the subject is to deepen the knowledge of students in analytical chemistry and to prepare them for solving analytical problems with use of instrumental methods. Emphasis is laid on the ability of students to select optimal methods and appropriate procedures with regard to the nature of the analysed object and to realize the scope and limitations of instrumental methods. The subject belongs to specialization Pharmaceutical analysis.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (23.07.2024)

Course completion requirements -

Credits of Special Methods of Instrumental Analysis are based on:

Reaching of the points from the credit test.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (23.07.2024)

Literature -

Recommended:

Skoog, Douglas A.. Skoog and West's fundamentals of analytical chemistry. [Belmont, Calif.]: Brooks/Cole Cengage Learning, 2013, 958 s. ISBN 978-1-285-05624-1.

Robinson, James W., Frame, Eileen M. Skelly Frame, George M.. Undergraduate instrumental analysis. New York: M. Dekker, 2005, 1079 s. ISBN 0-8247-5359-3.

Last update: prepocet_literatura.php (19.09.2024)

Syllabus -

Syllabus – Special Methods of Instrumental Analysis (lessons)

Introduction to special methods of instrumental analysis.
Selection of analytical technique following analyte type, sample matrix, analysis requirements. Survey of analytical techniques.
Luminescence analysis, fluorescence, phosphorescence, chemiluminescence, emission and excitation spectra, fluorimeters, spectrofluorimeters, qualitative and quantitative applications.
Atomic-absorption spectrophotometry, absorbing medium, resonance spectral lines, instrumentation, analytical applications. Atomic-fluorescence spectrometry.
Application of UV spectrphotometry for determination of analytes in mixtures.
Infrared spectroscopy and Raman spectroscopy. Application in pharmacetucial analysis, identification of active substances, packing materials, distribution of active substance in pharmaceutical formulation.
Pulse voltammetric methods. Difference pulse polarography. Stripping voltammetry.
Electromigration methods, electrophoretic mobility, principles of separation. Capillary isotachophoresis, capillary zone electrophoresis, instrumentation, analytical applications.
Isoelectric focusation, micellar electrokinetic chromatography. Capillary electrochromatography. Instrumentation, applications.
Modern trends in HPLC (van Deemter theory, new stacionary phases, fast chromatography, detection), Supercritical fluid chromatography.
Mass spectrometry, mass spectrum, principles, instrumentation, Analytical and Bioanalytical application of mass spectrometry.
Ion-exchange chromatography. Ion-exchange phenomena, cation and anion exchangers, ion-exchange equilibria, amino acid analyzers, molecular sieves, gel-permeation chromatography.
Automation of analytical procedures, titrators, types of analyzers and their applications.
Flow-injection analysis, sequential injection analysis, automation of analytical procedures, principles, instrumentation, applications. Sequential injection chromatography.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (23.07.2024)

Úvod do speciálních instrumentálních metod.
Výběr analytické techniky podle typu analytu, matrice vzorku, požadavků na analýzu. Přehled analytických technik.
Luminiscenční analýza, fluorescence, fosforescence, chemiluminiscence, emisní a excitační spektra, kvalitativní a kvantitativní využití.
Atomová absorpční spektrometrie, Atomová emisní spektrometrie, atomová fluorescenční spektrometrie, principy a analytické využití.
Spektrofotometrie v UV oblasti – možnosti stanovení více látek.
Infračervená spektroskopie a Ramanova spektroskopie. Aplikace ve farmaceutické analýze, identifikace účinných látek, obalových materiálů, distribuce účinné látky v léčivém přípravku.
Voltametrické metody. Diferenční pulzní voltametrie. Rozpouštěcí voltametrie. Amperometrické titrace. Iontově selektivní elektrody.
Elektromigrační metody, elektroforetická pohyblivost, principy. Kapilární izotachoforéza, Kapilární zónová elektroforéza, instrumentace, analytické využití.
Izoelektrická fokusace, micelární elektrokinetická chromatografie, kapilární elektrochromatografie. Instrumentace, analytické využití.
Současné trendy v HPLC (van Deemterova teorie, nové stacionární fáze, rychlá chromatografie, detekce), Superkritická fluidní chromatografie.
Hmotnostní spektrometrie, hmotnostní spektrum, principy, přístrojové vybavení, analytické a bioanalytické využití hmotnostní spektrometrie.
Iontovýměnná chromatografie. Metody výměny iontů, katexy, anexy, iontovýměnné rovnováhy, analyzátory aminokyselin, molekulová síta, gelová chromatografie.
Automatizace analytických postupů. Titrátory, robotické systémy, klinické analyzátory. Neseparační průtokové metody, průtoková injekční analýza.
Automatizace extrakčních postupů. Sekvenční injekční analýza. Sekvenční injekční chromatografie.

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (23.07.2024)

Learning outcomes

The subject Special Methods of Instrumental Analysis builds on the knowledge and skills acquired in the subjects: Physical Chemistry and Instrumental Methods.

After completing the subject, students will be able to use the following terms in the correct context towards the analytical evaluation of inorganic and organic substances using instrumental methods: electroanalytical methods - pulse voltammetry and dissolution voltammetry, amperometry and ion-selective electrodes, electromigration methods - capillary zone electrophoresis, micellar electrokinetic chromatography, isoelectric focusing, capillary isotachophoresis, capillary electrochromatography, modern trends in liquid chromatography, luminescence methods - fluorimetry, chemiluminescence, atomic absorption spectrophotometry, Raman spectrometry, mass spectrometry, automation - flow injection analysis, sequential injection analysis.

Learning outcomes:

Based on the acquired knowledge and skills, students will:

design a suitable instrumental technique for determining the content of a given organic substance describe the difference between individual optical/electroanalytical techniques with regard to selectivity, sensitivity of determination with application for the determination of analytes in real samples;
describe the difference between chromatographic and electromigration separation methods;
explain the principle of separation and describe basic electromigration methods;
explain modern trends in liquid chromatography, including sorbent geometry, column dimensions, UHPLC, detection techniques;
explain the principle of luminescence, the difference between fluorimetry and chemiluminescence, compare luminescent detection with spectrophotometric, describe basic chemiluminescence systems;
explain the principle of atomic absorption spectrometry and Raman spectrometry and their use for determining the content of analytes;
explain the possibilities of automation in low-pressure flow systems, describe the FIA/SIA system, the principle of measurement and evaluation of the content of determined substances

Last update: Sklenářová Hana, doc. PharmDr., Ph.D. (24.02.2025)