Basis of Harvesting and Preservation of Tissues for Transplantation - FG50003

• Title: Basis of Harvesting and Preservation of Tissues for Transplantation
• Guaranteed by: Tissue Bank (15-9995)
• Faculty: Faculty of Medicine in Hradec Králové
• Actual: from 2023
• Semester: summer
• Points: 0
• E-Credits: 1
• Examination process: summer s.:
• Hours per week, examination: summer s.:6/9, C [HS]
• Capacity: unlimited
• Min. number of students: 5
• 4EU+: yes
• Virtual mobility / capacity: yes / 20
• Key competences: 4EU+ Flagship 1
• State of the course: not taught
• Language: English
• Teaching methods: combined
• Teaching methods: combined
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: MUDr. Pavel Měřička, Ph.D.
• Incompatibility : FA0108088, FV065
• Pre-requisite : FG10012
• Interchangeability : FV065
• Is incompatible with: FV065
• Is interchangeable with: FV065

Annotation

Last update: MUDr. Pavel Měřička, Ph.D. (07.02.2022)

History of tissue banks, tissue donation - ethical and legal issues, the term of tissue establishment. Tissue harvest in living and deceased donors, cell and tissue graft processing. Principles of preservation methods, hypothermic preservation, deep freezing,cryopreservation,freeze-drying.Theory of cryoprotection. Safety and quality assurance in practice of tissue establishments. Contraindications of cell and tissue harvest, laboratory testing of donors and processed tissue grafts, importance of using clean room technology for safety of tissue transplantation. Single European Code for labeling cell and tissue products.Examples of use of preserved cells and tissues of human rigin in clinical practice. Advanced therapy medicinal products(ATMPs), manufacturing and clinical application. Cooperation of tissue establishments and pharmaceutical companies in development and manufacturing of ATMPs.

Requirements to the exam

Last update: MUDr. Pavel Měřička, Ph.D. (07.02.2022)

Credit

• Attendance in lectures, practical lessons and final seminar.
• Preparation of seminar thesis.

Electronoc course and seminar theses in case of cancellation of the standard teaching process 

Syllabus

Last update: MUDr. Pavel Měřička, Ph.D. (09.02.2022)

Lectures

1. History of tissue banks, tissue donation - ethical and legal issues (EU Directives and national legal norms of the Czech Republic), tissue harvests in living and deceased donors, cell and tissue graft processing, procurement establishment, tissue establishment, Compendium of tissue establishments of the EU member states (Mericka 2)
2. Principles of preservation methods, hypothermic preservation, deep freezing, cryopreservation, theory of cryoprotection, vitrification and its application in cell and tissue banking, freeze-drying, methods of organ preservation, organ bank. (Mericka 2)
3. Safety and quality assurance in practice of tissue establishments. Contraindications of tissue harvests, laboratory testing of donors and processed tissue grafts, importance of using clean room technology for safety of tissue transplantations,quality and safety assurance systems, Quality and safety in the practice of the organ bank. (Mericka 2)

Practical courses & seminars

Lecturers

• Pavel Mericka, M.D., Ph.D.
• RNDr.Jiri Gregor,Ph.D.
• Mgr. Miroslava Jandová
• RNDr. Barbora Honegrova,Ph.D.

Seminars

1. Application of tissue grafts and advanced therapy medicinal products in different medical disciplines - surgical disciplines, hematology and oncology. Methods of risk assesment and risk management in cell and tissue banking and transplantation.

Practical courses

1. Organisation of tissue harvests in living and deceased donors,donor eligibility - practical examples, demonstration of preservation methods and of difefernet types of tissue grafts, documentation on tissue harvest,processing  and application of tissue grafts. European Union Tissue Establishment Product list.Use of the Single European Code (SEC) for labeling of cell and tissue products. Use of clean room technology in processing of cell and tissue grafts, organ bank
2. Cryopreservation of cells and tissues, use of cell culture in clinical situations, advanced therapy medicinal products -manufacturing and clinical application. Cooperation between tissue establishments and pharmaceutical companies. Human milk bank, application of the system of Hazard Analysis and Critical Control Points in the practice of tissue and milk banks

Literature

Last update: MUDr. Pavel Měřička, Ph.D. (08.02.2022)

Compulsory literature

1. Warwick,R.M. et al. Tissue and Cell Donation. An essential guide. Willey-Blackwell,Oxford 2009 -selected chapters
2. Měřička,P,Navrátil,P.,Brandejs,D.,Lubomír Štěrba,Hana Straková,Barbora Honegrová:E-learning course: Donation and Harvest of Cells and Tissues. https://mefanet.lfhk.cuni.cz/articles.php?aid=69
3. Měřička,P.,Gregor,J.,Navrátil,P.,Jandová,M.,B. Honegrová, D.Brandejs: E-learning course:Low Temperature Preservation of Cells and Tissues .https://moodle.lfhk.cuni.cz/moodle2/course/view.php?id=2117

    

Recommend literature

1. Bopp.,K.F. Guide to safety and quality assurance for organs,tissues and cells Council of Europe Publishing,Strasbourg 2002
2. Collins,G.M.,Dubernard,J.M.,Persijn,G.G.,eds. Procurement,Preservation and Allocation of Vascularized Organs,Kluwer Academic Publ.,Dordrecht, Boston, London, 1997-selected chapters
3. Smit Sibinga,C.Th.,Das,P.C. Meryman,H.T., eds. Cryopreservation and Low Temperature Biology in Blood Transfusion,Kluwer Academic Publ. Dordrecht,Boston,London,1990-selected chapters
4. Měřička,P. et al.: Basis of Harvest and Preservation of Tissues. E-learning Course http://moodle.lfhk.cuni.cz/moodle2/course/view.php?id=660