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Course, academic year 2018/2019
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Biochemistry II - EA0903010
Title: Biochemie II
Guaranteed by: Ústav lékařské chemie a biochemie (14-70)
Faculty: Faculty of Medicine in Pilsen
Actual: from 2018 to 2019
Semester: winter
Points: 6
E-Credits: 6
Examination process: winter s.:
Hours per week, examination: winter s.:28/21, C+Ex [HS]
Capacity: unlimited / unlimited (unknown)
Min. number of students: unlimited
4EU+: no
Virtual mobility / capacity: no
Key competences:  
State of the course: taught
Language: English
Teaching methods: full-time
Teaching methods: full-time
Level:  
For type:  
Note: deregister from the exam date if a requisite was not fulfilled
for a repeated registration for Course credit + Exam, the student must have the Course credit exam passed
Guarantor: doc. MUDr. Jaromír Kotyza, CSc.
Teacher(s): doc. MUDr. Jaromír Kotyza, CSc.
MUDr. Radana Vrzáková
Co-requisite : EA0901002, EA0901010, EA0902001, EA0902003, EA0902400
Is co-requisite for: EA0905510, EA0906100, EA0905500, EA0905390, EA0906520
Is pre-requisite for: EA0906090
Files Comments Added by
download PA_2zub_17_18_zs.pdf Lectures 2017/18 MUDr. Vlastimil Kulda, Ph.D.
download SA_2zub_17_18_zs.pdf Practicals 2017/18 MUDr. Vlastimil Kulda, Ph.D.
Annotation -
Last update: MUDr. Vlastimil Kulda, Ph.D. (15.11.2017)
Metabolism of amino acids and a nucleic acids. Molecular diseases, DNA diagnostics, genetic engineering. Porphyrins, hemoglobin. Biochemistry of connective tissue, bones and teeth in particular. Xenobiochemistry. Fundamentals of clinical biochemistry, examination of body fluids.
Literature - Czech
Last update: MUDr. Vlastimil Kulda, Ph.D. (16.11.2017)

Obligatory literature:

Kotyza J. et al.: Clinical Chemistry and Enzymology - Laboratory Manual. Charles University, Faculty of Medicine in Pilsen, Plzen 1998.

Ferrier D.R. et al.: Lippincott Illustrated Reviews: Biochemistry (7th Edition). Wolters Kluwer, Philadelphia 2017.

 

Recommended literature:

Rodwell V.W. et al.: Harpers Illustrated Biochemistry (30th Edition). McGraw-Hill 2015.
Requirements to the exam
Last update: Vendulka Votípková (25.05.2018)

Semester Credit Requirements:
1. Attendance at seminaries and full attendance at laboratories are required. The missing laboratories must be substituted. Lab coats at laboratories are obligatory.
2. The laboratory protocols should be approved by the teacher in charge.
3. The subject matter is controlled by two tests. Three attempts only are allowed.

Final exam:
In order to register for the exam it is necessary to have obtained credits for both semesters and fulfill all the prerequisites. The requirements for the exam are given by the extent of lectures, seminars, and laboratories.
Final exam consists of the written and oral parts.
To pass the written test, 66 per cent of all questions should be correctly answered. Once a student passed the written test, he or she may advance to the oral exam. This consists of two parts. First is the exam from laboratory topics, where the student draws two topics (one from each semester). Then the student draws the main combination of three topics from curriculum presented in lectures. Student has the right for written preparation.
In case a student fails at the oral exam, he or she should repeat both the written and oral part. During the exam period, three exam attempts only are allowed.
Syllabus -
Last update: MUDr. Vlastimil Kulda, Ph.D. (16.11.2017)

Laboratories and Seminars:

Examination of blood (proteins, lipids, glucose, non-protein nitrogen compounds, bile pigments). Inner environment and acid-base balance.


Lectures:

Digestion of proteins, essential amino acids. Amino acid degradation, ammonia detoxification, the formation of urea. Metabolic reactions of aliphatic amino acids I. One-carbon units, the role of THFA.

Metabolic reactions of amino acids II. The formation of catecholamines and thyroid hormones. Arginine, NO.

Formation and degradation of purine and pyrimidine nucleotides. Uric acid and gout. Conversion to deoxynucleotides. DNA structure.

Replication and transcription of DNA. DNA repair. Structure of RNA (rRNA, tRNA).

Processing of mRNA, genetic code, proteosynthesis. Posttranslational modifications and sorting of proteins. Proteolytic systems.

DNA organization in chromatin, regulation of gene expression. Hormones and other regulatory molecules, signaling pathways. Programmed cell death (apoptosis).

Analysis of DNA and mRNA in diagnostics, PCR, restriction enzymes. Recombinant technologies.

DNA and RNA viruses. Retroviruses, HIV.

Porphyrin biosynthesis, heme and its degradation, hemoglobin. RBCs and oxygen radicals. Blood plasma proteins. Genesis of immunoglobulins. Biochemistry of blood clotting and clot dissolution.

Biochemistry of connective tissue, the formation of collagen, adhesive proteins. Metabolism of bone and tooth tissue.

Biology of the oral cavity, dental caries, calculus  and paradentosis.

Biochemistry of nervous tissue. Biochemistry and energetics of the muscle activity. Liver functions. Nutrition, malnutrition, starvation.
 
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