VP - Practical Course of Preparation of recombinant proteins as a basis for structural biology - B81679 (elective subject)

• Title: VP - Practical Course of Preparation of recombinant proteins as a basis for structural biology
• Guaranteed by: Department of Pediatrics and Inherited Metabolic Disorders First Faculty of Medicine Charles University and General University Hospital in Prague (11-00650)
• Faculty: First Faculty of Medicine
• Actual: from 2025 to 2025
• Semester: winter
• Points: 2
• E-Credits: 2
• Examination process: winter s.:
• Hours per week, examination: winter s.:0/18, C [HS]
• Extent per academic year: 18 [hours]
• Capacity: 6
• Min. number of students: 4
• 4EU+: no
• Virtual mobility / capacity: no
• State of the course: taught
• Language: English
• Teaching methods: full-time
• Explanation: Ing. Marie Zikánová, PhD
• Note: course can be enrolled in outside the study plan; enabled for web enrollment
• Guarantor: Ing. Marie Zikánová, Ph.D.
• Attributes: Lékařství; Teoretický předmět; Zubní lékařství

Annotation

This is an intensive three-day practical course focused on fundamental proteomic and biochemical techniques for recombinant protein preparation and analysis. The course is for highly motivated students of 3. to 6. year of general medicine studies, as well as PhD students. The course is conducted in Czech or English (or a combination of both), depending on the participants. Students will gain hands-on experience with recombinant protein expression in bacterial systems (E. coli), cell lysis and protein extraction, purification using affinity chromatography (IMAC), determination of protein concentration (Nanodrop, Bradford assay), and analysis of protein purity using SDS-PAGE. The course takes place at BIOCEV (Vestec) and is organized in small groups to ensure an individual approach. Minimum number of students: 3 Maximum number of students: 6

Last update: Špácová Marika, DiS. (27.03.2026)

Syllabus

Practical Course of Preparation of recombinant proteins as a basis for structural biology

Syllabus:

Preparation of recombinant proteins as a basis for structural biology (Ing. Ivan Mikula, Ing. Marie Zikánová, PhD)

Expression systems, Protein overexpression in the bacterial cells, Isolation and purification of proteins- different chromatographic purification techniques, Protein detection, Concentration measurement, "...what to do further with the protein?"

Info: [e-mail]

Last update: HAN03982 (16.10.2008)

Education plan

English

Schedule by date
Day	Date	Description	Teacher	Files	Note	Hodnocení
Monday	15.12.2025	First day of the practical course – Preparation of recombinant proteins as a basis for structural biology	Ing. Marie Zikánová, Ph.D.		This is a three-day course, and full attendance is mandatory.
Tuesday	16.12.2025	Second day of the practical course – Preparation of recombinant proteins as a basis for structural biology	Ing. Marie Zikánová, Ph.D.		This is a three-day course, and full attendance is mandatory.
Wednesday	17.12.2025	Third day of the practical course – Preparation of recombinant proteins as a basis for structural biology	Ing. Marie Zikánová, Ph.D.		This is a three-day course, and full attendance is mandatory.

Czech

Rozpis datumový
Den	Datum	Popis	Učitel	Soubory	Poznámka	Hodnocení
Pondělí	15.12.2025	First day of the practical course – Preparation of recombinant proteins as a basis for structural biology	Ing. Marie Zikánová, Ph.D.		This is a three-day course, and full attendance is mandatory.
Úterý	16.12.2025	Second day of the practical course – Preparation of recombinant proteins as a basis for structural biology	Ing. Marie Zikánová, Ph.D.		This is a three-day course, and full attendance is mandatory.
Středa	17.12.2025	Third day of the practical course – Preparation of recombinant proteins as a basis for structural biology	Ing. Marie Zikánová, Ph.D.		This is a three-day course, and full attendance is mandatory.