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Course, academic year 2022/2023
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Microbiology - B81148 (Zubní lékařství AP)
Title: Mikrobiologie
Guaranteed by: Institute of Immunology and Microbiology First Faculty of Medicine and General University Hospital in Prague (11-00351)
Faculty: First Faculty of Medicine
Actual: from 2019
Semester: summer
Points: 4
E-Credits: 4
Examination process: summer s.:
Hours per week, examination: summer s.:2/4, C+Ex [HT]
Extent per academic year: 90 [hours]
Capacity: unlimited
Min. number of students: unlimited
Virtual mobility / capacity: no
State of the course: taught
Language: English
Teaching methods: full-time
Explanation: Mgr. Kateřina Petříčková, Ph.D.; katerina.petrickova@lf1.cuni.czwhite coat for practicals only
Additional information: http://uim.lf1.cuni.cz
Note: prefer medical statement
Guarantor: prof. RNDr. Libuše Kolářová, CSc.
Comes under: Compulsory for Dentistry 2.y._22/23
Attributes: Teoretický předmět
Zubní lékařství
Pre-requisite : {splněna jedna z anatomií}, B81129, B83031
Is pre-requisite for: B81162, B81155, B81159, B81157, B83151, B83156, B81407
Annotation -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)
Basic knowledge of microbiology, special dental part of microbiology, virology, mycology and parasitology. Human patogens, their properties, diagnostics, immunization, antibiotics, antibacterial and virostatic therapy, vaccination. Microbiological apects of microflora of oral cavity, special dental microbiological profile. Methods of treatment. Practice: methods of sample collection of biological materials, methods of identification, stategy of antibacterial therapy - antibiotic centre, antibiotic police, interpretation of results of examination.
Aim of the course -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)

to teach future dentists, how to deal with infectious or contaminated material, decontamination, desinfection and sterilisation techniques, guidelines for aseptic work in a oral cavity area, the risks of infections concerning dental treatment, differential diagniosics of infectious diseases with manifestations in a oral cavity, basic strategies in antibiotic therapy. Basics of general and special medical microbiology including pathogenesis of the most important infections and anti-infection immunity mechanisms of the host.

Syllabus -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)

Basic properties of bacteria, their virulence factors, pathogenesis of infections. G+ and G- cocci, G+ and G- rods, Mycobacteria, Mycoplasmas, Chlamydiae, Rickettsiae, Spirochets. Basics of medical mycology and parasitology. Desinfection and sterilization, Antimicrobial drugs and strategy of therapy. General and special virology, pathogenesis of viral infections, immune mechanisms of the host, vaccination. HIV, HAV,HBV,HCV,HDV,HEV, Herpesviridae, Neurotropic viruses, Viral diarrhoeas. Respiratory Tract infections, Sexually transmitted infections. Infections with exanthemas and other skin and mucose manifestations. Oral Cavity microbial flora and infection processes. Diagnostic Techniques in medical microbiology.

Practicals  1  (one week, total 26 hours):

 Microscopy in medical bacteriology, mycology and parasitology. Native, fixed and stained slide. Staining techniques (Gram. Ziehl-Nielsen, Buri, Giemsa, immunofluorescence). Bacterial culture media, cultures and macroscopic morphology. Metabolic Tests (API, Pliva Staphytest, Streptotest, Enterotest) Toxin detection. Antigen detection, Typing. Antibiotic susceptibility tests (diffusion/disc tests, E-tests, MIC, MBC-testing) Serologic reactions( agglutination, precipitation, immunodiffusion, immunofluorescence, ELISA, immunoblot). Genome detection techniques - hybridisation, PCR. Horizontál electrophoresis, real-time PCR. Urine culture and interpreting of the test. Yeast culture (Sabouraud) and GT-test. Sample collection techniques and Kits, documentation, transport requirements. Disinfection and Sterilisation techniques and Sterility control. Blood cultures - proper taking of samples, culture systéme icl. BacTec, BactAlert. Anearobic Culture. Anaerostat.

 Practicals 2 (one week, total 26 hours):

 Students practisize collection of  their own samples: throat swab, nose swab, urine and transport them to the laboratory. During the week they follow algorithm of routine bacteriological laboratory work-flow hands-on. Additionaly they obtain coded patiens samples: urine, stool, vaginal swab and blood culture. They investigate each sample making different tests. At the end they have to provide results for each sample: Determined pathogen and its sensitivity to antibiotics Additionaly they perform Immunocard tests for different agents: viral or bacterial. They interpret serological tests and detect genome of EBV or CMV using PCR

Seminars (Attendance Compulsory):

  1. Rational antimicrobial therapy (3 hours)
  2. Prevention and therapy of viral diseases (3 hours)
Literature -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)

http://el.lf1.cuni.cz/mikrobiostoma/

Bednář M., Fraňková V., Schindler J., Souček A., Vávra J.: Lékařská mikrobiologie. Marvil, Praha, 1. vyd. 1996.

Votava M., Ondrovčík P.: Vybrané kapitoly z klinické mikrobiologie. Masarykova Universita, Brno, 2000.
Murray, Pfaller, Rosenthal eds. Medical Microbiology 5th edition, Mosby

Teaching methods -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)

Lectures and 2 one-week practical blocks and 5 compulsory seminars focused on microbiology of oral cavity and diagnostic and desinfection techniques.
Lectures and seminars: Inst. of Microbiol.Studničkova 7
Practical blocks: teaching laboratories of the Dept. Microbiology and immunology, Studničkova 7, Ground Floor, Back Entrance.

Entry requirements -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)

Prerequisites: B01129 Biochemistry and pathobiochemistry 2, B01401 Anatomy 2, B81402 Histology and embryology

 


DUE TO SAFETY REASONS, THE CAPACITY OF LABORATORIES HAS BEEN LIMITED TO MAXIMUM OF 14 STUDENTS.

A white coat and lab shoes required.

Assignment of the students in the practical weeks will be announced in advance in the SIS.

Changes of the assigned terms are allowed only due to serious and well documented reasons - illness, hospitalization, VISA issues.

Requirements to the exam -
Last update: Mgr. Kateřina Petříčková, Ph.D. (23.05.2019)

EXAMINATION OUESTIONS ON MICROBIOLOGY FOR STUDENTS OF DENTlSTRY

PART ONE: PRACTICALS

1. Dírect detection of infectious agent
2. Microscopy
3. Gram stain technique
4. Immunofluorescence
5. Media for bacterial cultures
6. Identification techniques for bacteria
7. Toxin detection and titration techniques
8. Disc diffusion test on antibiotic susceptibi1ity
9. MIC determination
10. MBC determination
11. Indirect detection of infectious agent
12. Serological reactions
13. ELlSA
14. CFR (Complement Fixing reaction)
15. Guidelines for proper collection of specimens - swabs
16. Guidelines for proper collection of blood cultures
17. Anarobic bacteria culture
18. Hybridization probes
19. Amplification techniques - PCR
20. Physical techniques for disinfection and Sterilisation
21. Autoclave - priciple and function
22. Parameters for sterilisation by saturated steam
23. Hot air sterilizer
24. Parameters for hot air sterilisation
25. Disinfection/ decontamination of hands
26. Techniques for decontamination oftools and materials
27. Techniques for control of sterilisation process
28. Techniques for control of area microbial purity
29. Guidelines for discarding of infectious and toxic waste
30. Bacteriological investigation of urine

PART TWO: General Microbiology

1. Basic properties of bacteria, structure of bacterial cell
2. Basic properties of viruses, virus structure
3. Basic properties of pathogenic funghi
4. Collection and transport of samples for microbiological examination
5. Laboratory diagnostics ofbacterial infections
6. Laboratory diagnostics of viral infections
7. Laboratory diagnosncs of parasitic infections
8. Laboratory diagnostics of mycotic infections
9. Surface structures ofbacterial cell
10. Bacterial cell wall
11. Genetic information in a bacterial cell
12. Disinfection and sterilisation, decontamination, asepsis
13. Antibiotics - mechanisms of action
14. Resistence to antibiotic, development and prevention
15. Guidelines for rational antibiotic use for treatment
16. Prophylactic use of antibiotics
17. Antimycotics, Antivirals, Antiparasitics
18. Pathogenicity and virulence ofbacteria
19. Nonnal microbial flora ofhumans
20. Adherence ofbacteria, infections ofmucoid membranes
21. Bacterial toxins, toxinoses
22. Endotoxin
23. Pathogenesis of viral infections
24. Anaerobic infections
25. Cellular immune mechanisms
26. Humoral immune mechanisms
27. Principles of immunisation against infectious diseases
28. Types ofvaccÍnes for active immunisation
29. Passive immunisation
30. Nosocomial (hospital) infections

PART THREE: Specialized microbiology

1. Oral Cavity Microbial Flora
2. Oral Cavity Anaerobic Flora
3. Dental plaque
4. Caries - role of microorganisms in development
5. Parodontitis,. parodontosis
6. Odontogenic bacterial infections
7. Bacterial infections of the oral cavity
8. Viral infections of the oral cavity
9. Fungal infections ofthe oral cavity
10. Exanthemas and enanthemas of infectious origin
11. Agents causing invasive and inflammatory diseases of the GIT
12. Agents causing toxic infections ofthe GIT
13. Bacteria causing infections of upper respiratory tract
14. Bacteria infecting lower respiratory tract
15. Microbiology of Tuberculosis
16. Viral respiratory infections
17. Mycotic and parasitic infections of resp. tract
18. Agents infecting urinary tract
19. Agents of sexua1ly transmittable diseases
20. HIV and AIDS
21. How to treat an HIV -positive patient
22. Agents causing meningitis and encepha1itis
23. Wound infections
24. Sepsis and septic shock
25. Hepatitis viruses
26. Anaerobic infections
27. Agents acting in Sinusitis
28. Syphilis and manifestations in the oral cavity
29. Risks of infection following tooth extraction and other dental surgeries
30. Actinomycets and actinomycoses.

 
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