SubjectsSubjects(version: 873)
Course, academic year 2012/2013
Plant cell and molecular biology - a practical course - MB130C34
Title: Praktikum z buněčné a molekulární biologie rostlin
Guaranteed by: Department of Experimental Plant Biology (31-130)
Faculty: Faculty of Science
Actual: from 2012 to 2012
Semester: summer
E-Credits: 2
Examination process: summer s.:
Hours per week, examination: summer s.:0/1 C [weeks/semester]
Extent per academic year: 1 [weeks]
Capacity: unlimited
Min. number of students: unlimited
State of the course: taught
Language: Czech
Additional information:
Guarantor: prof. RNDr. Viktor Žárský, CSc.
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Annotation -
Last update: RNDr. Hana Konrádová, Ph.D. (18.03.2019)
The course includes practical examples and theoretical background of a selection of modern methods of plant cell and molecular biology, in particular the techniques employed to study gene expression in plant material, transient expression of introduced genes in plant cells and protoplasts, and construction and analysis of transgenic plants.
The course is intended mainly for Masters and PhD students whose experimental interests include some of the methods taught.
Literature - Czech
Last update: RNDr. Hana Konrádová, Ph.D. (18.03.2019)

Syllabus -
Last update: RNDr. Hana Konrádová, Ph.D. (18.03.2019)

Practical tasks :

1. Detection of the cytoskeleton in cultured plant cells (immunofluorescence microscopy, Western blot)

2. Expression and affinity purification of a bacterially expressed plant protein.

3. Transient expression of a GFP-tagged protein using the tobacco leaf infiltration method.

4. Transient expression of a gene introduced into tobacco protoplasts (and detection of a GFP-tagged gene product).

5. Arabidopsis transformation (in situ infiltration by Agrobacterium, transgene selection).

6. Detection of inducible gene expression in plant cells using the reporter gene approach.

7. Transgenic plant detection using PCR.

8. In silico cloning and mutagenesis - introduction into basic bioinformatic tools.

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