Regulace exprese S10 operonu Escherichia coli.

• Thesis title in Czech: Regulace exprese S10 operonu Escherichia coli.
• Thesis title in English: Regulation of S10 operon expression in Escherichia coli.
• Academic year of topic announcement: 2009/2010
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Genetics and Microbiology (31-140)
• Supervisor: RNDr. Martin Pospíšek, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 07.01.2010
• Date of assignment: 25.11.2010
• Date of electronic submission: 08.06.2010
• Date of proceeded defence: 08.06.2010
• Opponents: RNDr. Irena Lichá, CSc.

Preliminary scope of work

Ribozóm?Gram-negativní bakterie Escherichia coli se skládá ze tří různých molekul rRNA a z?53 proteinů. Jejich syntéza v exponenciálně rostoucích buňkách je koordinovaně řízena. Operon S10 kóduje 11 z 53 ribozomálních proteinů. Regulace exprese proteinů S10 operonu probíhá na úrovni translace mechanismem autogenní kontroly, běžným u ribozomálních proteinů v E. coli, při kterém jeden z proteinů kódovaných daným operonem má nejen strukturní ale i regulační funkci, ale také na úrovni transkripce mechanismem atenuace

Preliminary scope of work in English

A Gram-negative Escherichia coli ribosome particle is comprised of 3 different rRNA molecules and 53 proteins. Their synthesis in exponentially growing cultures is coordinated. S10 operon codes for 11 of 53 ribosomal proteins. The expression of ribosomal proteins clustered in S10 operon is regulated autogenously at the level of translation, as is common with other E. coli ribosomal protein operons, where one of the proteins in the operon has both structural and regulatory functions, but also at the level of transcription by way of premature transcription termination ? attenuation.