Plant exocyst subunits EXO70 in autophagy and secretion

• Thesis title in Czech: Podjednotky EXO70 rostlinného komplexu exocyst v autufagii a sekreci
• Thesis title in English: Plant exocyst subunits EXO70 in autophagy and secretion
• Key words: exocyst; sekrece; autofagie; nekonvenční sekrece bílkovin; Arabidopsis; transport a ukládání kovů u rostlin
• English key words: exocyst; secretion; autophagy; unconventional protein secretion; Arabidopsis; metal transport and deposition in plants
• Academic year of topic announcement: 2022/2023
• Thesis type: dissertation
• Thesis language: angličtina
• Department: Department of Experimental Plant Biology (31-130)
• Supervisor: prof. RNDr. Viktor Žárský, CSc.
• Author: hidden - assigned by the advisor
• Date of registration: 12.10.2022
• Date of assignment: 12.10.2022
• Advisors: prof. RNDr. Fatima Cvrčková, Dr.

Guidelines

Plant cell biology ; Plant developmental biology; Plant Physiology (as an alternative)

References

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Preliminary scope of work

Před 10 lety byla popsána údajná nová organela v rostlinných buňkách - EXPO. Na její tvorbě se podílí také isoforma podjednotky exocystu EXO70E2. Cílem této disertace je prověřit mechanismy působení EXO70E2 a jejich možný vztah k autofagii.

Preliminary scope of work in English

Ten years ago, a plant-specific endomembrane compartment – the EXPO (Exocyst-Positive
Organelle) was described. Markers for the Golgi, early or late endosome do not colocalize with
double membrane EXPO; it is not induced by starvation, does not co-localize with
autophagosomes and does not fuse with the vacuole. Instead, it fuses with the plasma
membrane, releasing a single membrane vesicle into the cell wall, comprising a putative new EXPO
exocytotic pathway. Further studies of this pathway focused on EXO70E2 subunit of the
Arabidopsis exocyst complex. However, over the years we found that original EXPO features
are mostly related to artefacts connected with exocyst subunits overexpression, and - most
importantly - for the EXO70B1 isoform we proved unequivocally direct link to autophagy. In this
project we will focus on major EXPO forming protein EXO70E2 and its closest homolog
EXO70E1, test its direct connection to the autophagy pathway, address its currently unknown
biological role, and clarify outstanding questions and problems of the EXPO compartment
concept in plant cells.