Vymezení druhových hranic v sekci Candidi rodu Aspergillus

• Thesis title in Czech: Vymezení druhových hranic v sekci Candidi rodu Aspergillus
• Thesis title in English: Species delimitation in Aspergillus section Candidi
• Key words: Aspergillus candidus, druhová delimitace, mnohogenová fylogeneze, morfologie, fyziologie, vnitrodruhová variabilita
• English key words: Aspergillus candidus, species delimitation, multigene phylogeny, morphology, physiology, intraspecific variability
• Academic year of topic announcement: 2019/2020
• Thesis type: diploma thesis
• Thesis language: čeština
• Department: Department of Botany (31-120)
• Supervisor: MUDr. Mgr. Vít Hubka, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 13.11.2019
• Date of assignment: 13.11.2019
• Date of electronic submission: 25.04.2022
• Date of proceeded defence: 25.05.2022
• Opponents: Mgr. Miroslav Caboň, Ph.D.

References

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Preliminary scope of work

Aspergillus sekce Candidi momentálně zahrnuje sedm druhů, nicméně podstatná část druhové diverzity je podle předběžných dat zatím nepopsána. Nejznámějším zástupcem je Aspergillus candidus, který znehodnocuje potraviny a krmivo, a produkuje mnoho bioaktivních látek a enzymů s potenciálním využitím v biotechnologiích a průmyslu. Řada druhů má také klinický význam a způsobuje onemocnění člověka. Zástupci sekce Candidi jsou si morfologicky velmi podobní a jejich identifikace na úroveň druhu je náročná i s použitím molekulárních metod, protože druhové hranice nejsou jasně definované. Přesto je identifikace důležitá z hlediska druhově specifické produkce enzymů, sekundárních metabolitů a různých citlivostí druhů k antimykotikům. Tento projekt si klade za cíl provést taxonomickou revizi druhových hranic sekce Candidi s použitím polyfázického přístupu. Prvotní hypotéza o druhových hranicích bude vytvořena na základě moderních delimitačních metod založených na modelu mnohodruhové koalescence a dále podpořena komplexní analýzou morfologie a fyziologie.

Preliminary scope of work in English

Aspergillus section Candidi includes seven species. However, according to the preliminary data, a substantial part of the diversity remains to be discovered. Aspergillus candidus is the most well-known member, which causes food and feed spoilage and produces many bioactive compounds and enzymes with biotechnological and industrial potential. Several species are clinically relevant and cause a wide range of human infections. Section Candidi members show uniform morphology and their identification to a species level is difficult even with the use of molecular methods, because the species boundaries are poorly resolved. However, their identification is important as different species produces specific secondary metabolite and enzyme spectra and have different antifungal susceptibility patterns. This project aims to provide a revision of species boundaries with the use of polyphasic approach. The primary hypothesis concerning the species limits will be created with the use of up-to-date delimitation method based on the multispecies coalescence model. Additionally, a complex analysis of morphology and physiology.