Separace proteinů gradientovou chromatofokusací

• Thesis title in Czech: Separace proteinů gradientovou chromatofokusací
• Thesis title in English: Gradient chromatofocusing for separation of proteins
• Key words: gradientová chromatofokusace, separace proteinů, vysokoúčinná kapalinová chromatografie
• English key words: gradient chromatofocusing, protein separation, high performance liquid chromatography
• Academic year of topic announcement: 2014/2015
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Physical and Macromolecular Chemistry (31-260)
• Supervisor: doc. RNDr. Květa Kalíková, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 12.08.2014
• Date of assignment: 28.11.2014
• Date of electronic submission: 20.05.2015
• Date of proceeded defence: 08.06.2015
• Opponents: RNDr. Martina Riesová, Ph.D.
• Advisors: Mgr. Tereza Šlechtová, Ph.D.

Preliminary scope of work

Testování dvou iontově výměnných kolon (anexů) pro separaci vybraných proteinů metodou gradientové chromatofokusace. Optimalizace gradientu a složení mobilní fáze.

Preliminary scope of work in English

Separation of selected proteins using two columns for anion-exchange chromatography by gradient chromatofocusing method. Optimization of gradient and mobile phase composition.