Thesis (Selection of subject)

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Zastoupení komponent ATP synthasomu v různých tkáních potkana a u pacientů s defektem ATP synthasy

Thesis title in Czech:	Zastoupení komponent ATP synthasomu v různých tkáních potkana a u pacientů s defektem ATP synthasy
Thesis title in English:	The content of components of ATP synthasome in different rat tissues and in patients with defects in ATP synthase
Key words:	ATP synthasa, ATP synthasom, ANT, hnědá tuková tkáň, elektroforéza,OXPHOS, PiC, solubilizace, superkomplexy, TMEM70
English key words:	ATP synthase, ATP synthasome, ANT, BAT, electrophoresis, OXPHOS, PiC,solubilization, supercomplexes, TMEM70
Academic year of topic announcement:	2010/2011
Thesis type:	diploma thesis
Thesis language:	čeština
Department:	Department of Cell Biology (31-151)
Supervisor:	MUDr. Josef Houštěk, CSc., DrSc.
Author:	hidden - assigned by the advisor, čeká na schválení garantem
Date of registration:	01.11.2010
Date of assignment:	28.01.2011
Date of electronic submission:	27.08.2012
Date of proceeded defence:	13.09.2012
Opponents:	doc. RNDr. Martin Kalous, CSc.

Preliminary scope of work

Izolace mitochondrií ze tkání potkana a z buněk pacientů (defekt v ATP synthase) a kontrol, detekce a kvantifikace komponent fosforylačního aparátu v mitochondriích (SDS-PAGE/WB), spektrofotometrické měření aktivit enzymů OXPHOS, solubilizace membránových proteinů detergenty (digitonin, DDM), studium vzájemných interakcí mezi komponentami fosforylačního aparátu (různé typy nativních elektroforéz, 2DE, MSA)

Preliminary scope of work in English

Isolation of mitochondria from rat tissues and cells from patients (different deffects in ATP synthase) and control cells, detection and quantification the components of phosphorylative apparatus (SDS-PAGE/WB), spectrophotometric measurement of OXPHOS enzyme activities, solubilization of membrane proteins by detergents (digitonin, DDM), analysis of interactions between the components of phosphorylative apparatus (different types of native electrophoresis, 2DE, MSA)