The secretory vesicles tethering complex exocyst and the auxin transport polarization

• Thesis title in Czech: Poutací komplex exocyst a polarizovaný transport auxinu
• Thesis title in English: The secretory vesicles tethering complex exocyst and the auxin transport polarization
• Key words: exocyst, auxin
• English key words: exocyst, auxin
• Academic year of topic announcement: 2003/2004
• Thesis type: dissertation
• Thesis language: angličtina
• Department: Department of Experimental Plant Biology (31-130)
• Supervisor: prof. RNDr. Viktor Žárský, CSc.
• Author: hidden - assigned by the advisor
• Date of registration: 09.03.2010
• Date of assignment: 30.05.2011
• Date of electronic submission: 08.06.2011
• Date of proceeded defence: 27.09.2011
• Opponents: prof. RNDr. František Baluška, Ph.D.

Preliminary scope of work

A Změřit transport radioaktivně značené IAA a porovnat aktivitu v auxinovém maximu s využitím DR5 reportéru mezi WT a exo70A1.
B Objasnit zapojení EXO70A1 a dalších podjednotek exocystu v lokalizaci a recyklaci PIN proteinů-výtokových přenašečů auxinu.
C Zjistit funkční specificitu EXO70A1 pro transport a recyklaci PIN proteinů.
D Určit lokalizaci EXO70A1 a dalších podjednotek exocystu v závislosti na fosfolipidovém složení plazmatické membrány.
E Metodou koimunoprecipitace hledat interagující partnery podjednotky EXO70A1.

Preliminary scope of work in English

A To measure and compare radioactively labeled IAA transport and activity in IAA maxima using DR5 reporter between WT and exo70A1.

B To analyse possible involvement of EXO70A1 along with other exocyst subunits in auxin efflux carriers (PINs) localization and recycling.

C To analyse specificity of EXO70A1 for PIN recycling.

D To address the EXO70A1 and some other exocyst subunits localization in respect to membrane lipid composition.

E To study direct or indirect interactions between EXO70A1 and other proteins using co-immunoprecipitation.