Syntéza 5´-UTR varianty Var14 genu GCPII jako standardu pro RT-PCR

• Thesis title in Czech: Syntéza 5´-UTR varianty Var14 genu GCPII jako standardu pro RT-PCR
• Thesis title in English: Synthesis of the Var14 variant of 5´-UTR of GCPII gene as a standard for RT-PCR
• Key words: 5´UTR, dvoukroková PCA, pUC19, RT-PCR, GCPII
• English key words: 5'UTR, two-step PCA, pUC19, RT-PCR, GCPII
• Academic year of topic announcement: 2010/2011
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Biochemistry (31-250)
• Supervisor: RNDr. Marek Ingr, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 04.11.2009
• Date of assignment: 24.01.2011
• Date of electronic submission: 03.06.2011
• Date of proceeded defence: 14.06.2011
• Opponents: RNDr. Věra Černá, Ph.D.

Preliminary scope of work

syntéza sekvence 5´-UTR genu GCPII varianty Var14, příprava expresního vektoru, příprava standardu pro RT-PCR a měření kalibrační křivky

Preliminary scope of work in English

synthesis of 5'-UTR sequence of the gene variants GCPII Var14, preparation of expression vector, preparing a standard for RT-PCR and measurement of calibration curves