Factors influencing T cell surface nanotopography in health and disease
| Thesis title in Czech: | Fyziologické a patologické faktory ovlivňující povrchovou nanotopografii T buněk |
|---|---|
| Thesis title in English: | Factors influencing T cell surface nanotopography in health and disease |
| Key words: | T buňky, stresové faktory, membránové molekuly, povrchová morfologie, 3D zobrazování, mikroskopie s vysokým rozlišením |
| English key words: | T cells, stress factors, membrane molecules, surface nanotopography, 3D imaging, super-resolution microscopy |
| Academic year of topic announcement: | 2022/2023 |
| Thesis type: | dissertation |
| Thesis language: | angličtina |
| Department: | Department of Cell Biology (31-151) |
| Supervisor: | Mgr. Marek Cebecauer, Ph.D. |
| Author: | hidden - assigned by the advisor |
| Date of registration: | 03.10.2022 |
| Date of assignment: | 03.10.2022 |
| Guidelines |
| Cell biology Seeing is believing I. and II. |
| Preliminary scope of work |
| viz anglickou verzi |
| Preliminary scope of work in English |
| The accumulation of critical receptors at the tips of membrane protrusions on the cell surface was recently discovered by our group and others (Franke et al. 2022). Such nanoscopic organisation of receptors is key for regulation of signalling events controlling complex cellular processes. In this project, we will study how stress factors (e.g., temperature or oxidative burst) affect three-dimensional organisation of molecules on the surface of human leukocytes. The effect of metabolic inflammation factors such as leukotrienes and bradykinins, which are lipophilic compounds, will be tested separately and in combination with physical stress factors. This research will help to understand the impact of physical stress and in combination with inflammation on cell morphology and key cellular processes. The work will include imaging of receptors and associated effector molecules on the surface of stressed T cells. The imaging approaches will include a panel of advanced fluorescence and super-resolution microscopy techniques, which will be complemented by flow cytometry and some basic biochemistry methods to determine the impact of stress on T-cell function. Franke C; Chum T; Kvicalova Z; GlatzovaD; Gentsch GJ; Rodriguez A; Helmerich DA.; Herdly L; Mavila H; Frank O; Brdicka T; van de Linde S and Cebecauer M. Approach to map nanotopography of cell surface receptors. Comms Biol. 5: 218 (2022); DOI: 10.1038/s42003-022-03152-y |