Příprava DNA vazebné domény transkripčního faktoru FOXK1
| Thesis title in Czech: |
Příprava DNA vazebné domény transkripčního faktoru FOXK1 |
| Thesis title in English: |
Preparation of the transcription factor FOXK1 DNA binding domain |
| Key words: |
transkripční faktory, DNA vazebná doména, oligonukleotid, trojrozměrná struktura,
karcinogeneze |
| English key words: |
transcription factors, DNA binding domain, oligonucleotide, three-dimensional
structure, carcinogenesis |
| Academic year of topic announcement: |
2020/2021 |
| Thesis type: |
Bachelor's thesis |
| Thesis language: |
čeština |
| Department: |
Department of Biochemistry (31-250) |
| Supervisor: |
RNDr. Petr Novák, Ph.D. |
| Author: |
hidden - assigned by the advisor |
| Date of registration: |
09.11.2020 |
| Date of assignment: |
10.11.2020 |
| Date of electronic submission: | 17.06.2021 |
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| Date of proceeded defence: |
27.07.2021 |
| Opponents: |
Mgr. Dalibor Košek, Ph.D. |
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| Advisors: |
Ing. Aleš Hnízda, Ph.D. |
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| Preliminary scope of work |
| Rekombinatní příprava DNA vazné domený transkripčního faktoru v E. coli, purifikace rekombinatního proteinu, afinitní chromatografie, ionexová chromatografie, gelová filtrace, SDS elektroforesa, ionizace nativním elektrosprejem, nativní elektroforesa |
| Preliminary scope of work in English |
| Recombinant DNA-binding transcription factor domain preparation in E. coli, recombinant protein purification, affinity chromatography, ion-exchange chromatography, gel filtration, SDS electrophoresis, native electrospray ionization, native electrophoresis |
