Exocyst a MLO proteins in cell wall modifications.
| Thesis title in Czech: | Bílkoviny exocystu a MLO v modifikacích buněčné stěny. |
|---|---|
| Thesis title in English: | Exocyst a MLO proteins in cell wall modifications. |
| Key words: | exocyt; MLO; cell wall; callose; trichome; defence; Arabidopsis |
| English key words: | exocyst; MLO; buněčná stěna; kalóza; trichom; obrana; Arabidopsis |
| Academic year of topic announcement: | 2019/2020 |
| Thesis type: | dissertation |
| Thesis language: | angličtina |
| Department: | Department of Experimental Plant Biology (31-130) |
| Supervisor: | prof. RNDr. Viktor Žárský, CSc. |
| Author: | hidden - assigned and confirmed by the Study Dept. |
| Date of registration: | 15.10.2019 |
| Date of assignment: | 16.10.2019 |
| Confirmed by Study dept. on: | 16.10.2019 |
| Advisors: | Mgr. Peter Sabol, Ph.D. |
| Guidelines |
| MB130P13E - Plant physiology MB120P172 - Plant Epigenetics |
| References |
| Sekereš J, Pejchar P, Šantrůček J, Vukašinović N, Žárský V, Potocký M. Analysis of exocyst subunit EXO70 family reveals distinct membrane polar domains in tobacco pollen tubes. Plant Physiol. 173: 1659-1675, doi:10.1104/pp.16.01709, 2017 Pecenková T, Markovic V, Sabol P, Kulich I, Žárský V. Exocyst and autophagy-related membrane trafficking in plants. J. Exp. Bot. Dec 18;69(1):47-57. doi: 10.1093/jxb/erx363. 2017. Kulich I, Vojtíková Z, Sabol P, Ortmannová J, Neděla V, Tihlaříková E, Žárský V. EXO70H4 has a specific role in callose synthase secretion, essential for silica accumulation. Plant Physiol. 2018 Jan 4. pii: pp.01693.2017. doi: 10.1104/pp.17.01693. [Epub ahead of print] Sabol P, Kulich I, Žárský V. RIN4 recruits the exocyst subunit EXO70B1 to the plasma membrane. J. Exp. Bot. 68(12):3253-3265, doi: 10.1093/jxb/erx007, 2017. Vukašinović N, Oda Y, Pejchar P, Synek L, Pečenková T, Rawat A, Sekereš J, Potocký M, Žárský V. Microtubule-dependent targeting of the exocyst complex is necessary for xylem development in Arabidopsis. New Phytol. 213(3):1052-1067, 2017 - doi: 10.1111/nph.14267., 2017. |
| Preliminary scope of work |
| Navzdory velkému pokroku v poznání regulace buněčné polarity u rostlin je dosud pochopena jen zlomkovitě. V rámci projektu budeme studovat možnou spolupráci bílkovin komplexu exocyst a MLO při biogenezi sekundární buněčné stěny trichomů Arabidopsis s v obraně. Tento projekt probíhá ve spolupráci s kolegy z Univerzity v Cáchách podporované společným projektem DFG-GAČR. |
| Preliminary scope of work in English |
| Despite a considerable progress in uncovering fundamental molecular processes involved in the regulation of eukaryotic cell polarity, the understanding of this phenomenon in plants is still fragmentary at best. In collaboration with German colleagues we found the possibility of an unexpected co-ordinated involvement of exocyst and class of plant defence proteins in the regulation of targeted exocytosis. Exocyst is an evolutionarily conserved cytoplasmic secretory vesicle-tethering complex implicated in the targeting of secretory pathway components to specific plasma membrane (PM) domains; on the contrary, the molecular function of this class of plant defence proteins remains unknown. Unpublished observations in our labs revealed that Arabidopsis loss-of-function mutants in these defence proteins and the exocyst have almost identical phenotypic defects in the biogenesis of secondary cell walls. We aim to elucidate currently unknown molecular mechanism(s) of these defence proteins activity in the context of exocyst function in polarized exocytosis. We will collaborate with our German colleagues to address the presumed interplay of these proteins in defence as well as secondary wall biological contexts. Our efforts should substantially contribute to the understanding of the enigmatic molecular mechanism of these defence proteins. |