Časné změny exprese eNOS a ICAM-1 v aortě myší po podávání vysokotukové diety.

• Thesis title in Czech: Časné změny exprese eNOS a ICAM-1 v aortě myší po podávání vysokotukové diety.
• Thesis title in English: Early changes in eNOS and ICAM-1 expression in mice aorta after administration of high fat diet.
• Academic year of topic announcement: 2015/2016
• Thesis type: diploma thesis
• Thesis language: čeština
• Department: Department of Biological and Medical Sciences (16-16150)
• Supervisor: prof. PharmDr. Petr Nachtigal, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 09.11.2015
• Date of assignment: 24.01.2017
• Date and time of defence: 29.05.2017 08:00
• Date of electronic submission: 10.05.2017
• Date of proceeded defence: 29.05.2017
• Opponents: PharmDr. Kateřina Blažíčková, Ph.D.

Guidelines

1. Rešerše odborné literatury
2. Analyza vybranných markerů ve stěně cévy
3. Praktické provedení daných úkolů
4. Zhodnocení
5. Diskuse

References

[1] Valbuena-Diez, A. C., Blanco, F. J., Oujo, B., Langa, C., et al., Oxysterol-Induced Soluble Endoglin Release and Its Involvement in Hypertension. Circulation 2012.
[2] Nachtigal, P., Zemankova Vecerova, L., Rathouska, J., Strasky, Z., The role of endoglin in atherosclerosis. Atherosclerosis 2012, 224, 4-11.
[3] Li, X., van der Meer, J. J., van der Loos, C. M., Ploegmakers, H. J., et al., Microvascular endoglin (CD105) expression correlates with tissue markers for atherosclerotic plaque vulnerability in an ageing population with multivessel coronary artery disease.Histopathology 2012, 61, 88-97.
[4] Strasky, Z., Vecerova, L., Rathouska, J., Slanarova, M., et al., Cholesterol Effects on Endoglin and Its Downstream Pathways in ApoE/LDLR Double Knockout Mice. Circ J 2011, 75, 1747-1755.
[5] Rathouska, J., Vecerova, L., Strasky, Z., Slanarova, M., et al., Endoglin as a possible marker of atorvastatin treatment benefit in atherosclerosis. Pharmacol Res 2011, 64, 53-59.
[6] Ikemoto, T., Hojo, Y., Kondo, H., Takahashi, N., et al., Plasma endoglin as a marker to predict cardiovascular events in patients with chronic coronary artery diseases. Heart Vessels 2011.
[7] Tian, F., Zhou, A. X., Smits, A. M., Larsson, E., et al., Endothelial cells are activated during hypoxia via endoglin/ALK-1/SMAD1/5 signaling in vivo and in vitro. Biochem Biophys Res Commun 2010, 392, 283-288.
[8] Shyu, K. G., Wang, B. W., Chen, W. J., Kuan, P., Hung, C. R., Mechanism of the inhibitory effect of atorvastatin on endoglin expression induced by transforming growth factor-beta1 in cultured cardiac fibroblasts. Eur J Heart Fail 2010, 12, 219-226.
[9] Perez-Gomez, E., Del Castillo, G., Juan Francisco, S., Lopez-Novoa, J. M., et al., The role of the TGF-beta coreceptor endoglin in cancer. ScientificWorldJournal 2010, 10, 2367-2384.
[10] Lopez-Novoa, J. M., Bernabeu, C., The physiological role of endoglin in the cardiovascular system. Am J Physiol Heart Circ Physiol 2010, 299, H959-974.
[11] Kapur, N. K., Heffernan, K. S., Yunis, A. A., Parpos, P., et al., Usefulness of soluble endoglin as a noninvasive measure of left ventricular filling pressure in heart failure. Am J Cardiol 2010, 106, 1770-1776.
[12] Hawinkels, L. J., Kuiper, P., Wiercinska, E., Verspaget, H. W., et al., Matrix metalloproteinase-14 (MT1-MMP)-mediated endoglin shedding inhibits tumor angiogenesis. Cancer Res 2010, 70, 4141-4150.
[13] Blazquez-Medela, A. M., Garcia-Ortiz, L., Gomez-Marcos, M. A., Recio-Rodriguez, J. I., et al., Increased plasma soluble endoglin levels as an indicator of cardiovascular alterations in hypertensive and diabetic patients. BMC Med 2010, 8, 86.
[14] Nachtigal, P., Vecerova, L., Pospisilova, N., Micuda, S., et al., Endoglin co-expression with eNOS, SMAD2 and phosphorylated SMAD2/3 in normocholesterolemic and hypercholesterolemic mice: an immunohistochemical study. Histol Histopathol 2009, 24, 1499-1506.
[15] Nachtigal, P., Pospisilova, N., Vecerova, L., Micuda, S., et al., Atorvastatin Increases Endoglin, SMAD2, Phosphorylated SMAD2/3 and eNOS Expression in ApoE/LDLR Double Knockout Mice. J Atheroscler Thromb 2009, 16, 265-274.
[16] Bot, P. T., Hoefer, I. E., Sluijter, J. P., van Vliet, P., et al., Increased expression of the transforming growth factor-beta signaling pathway, endoglin, and early growth response-1 in stable plaques. Stroke 2009, 40, 439-447.
[17] Velasco, S., Alvarez-Munoz, P., Pericacho, M., Dijke, P. T., et al., L- and S-endoglin differentially modulate TGFbeta1 signaling mediated by ALK1 and ALK5 in L6E9 myoblasts. J Cell Sci 2008, 121, 913-919.
[18] ten Dijke, P., Goumans, M. J., Pardali, E., Endoglin in angiogenesis and vascular diseases. Angiogenesis 2008, 11, 79-89.
[19] Cruz-Gonzalez, I., Pabon, P., Rodriguez-Barbero, A., Martin-Moreiras, J., et al., Identification of serum endoglin as a novel prognostic marker after acute myocardial infarction. J Cell Mol Med 2008, 12, 955-961.
[20] Blanco, F. J., Grande, M. T., Langa, C., Oujo, B., et al., S-endoglin expression is induced in senescent endothelial cells and contributes to vascular pathology. Circ Res 2008, 103, 1383-1392.
[21] Blaha, M., Cermanova, M., Blaha, V., Jarolim, P., et al., Elevated serum soluble endoglin (sCD105) decreased during extracorporeal elimination therapy for familial hypercholesterolemia. Atherosclerosis 2008, 197, 264-270.
[22] Scherner, O., Meurer, S. K., Tihaa, L., Gressner, A. M., Weiskirchen, R., Endoglin differentially modulates antagonistic transforming growth factor-beta1 and BMP-7 signaling. J Biol Chem 2007, 282, 13934-13943.
[23] Santibanez, J. F., Letamendia, A., Perez-Barriocanal, F., Silvestri, C., et al., Endoglin increases eNOS expression by modulating Smad2 protein levels and Smad2-dependent TGF-beta signaling. J Cell Physiol 2007, 210, 456-468.
[24] Lopez-Novoa, J. M., Soluble endoglin is an accurate predictor and a pathogenic molecule in pre-eclampsia. Nephrol Dial Transplant 2007, 22, 712-714.
[25] Bernabeu, C., Conley, B. A., Vary, C. P., Novel biochemical pathways of endoglin in vascular cell physiology. J Cell Biochem 2007, 102, 1375-1388.
[26] Venkatesha, S., Toporsian, M., Lam, C., Hanai, J., et al., Soluble endoglin contributes to the pathogenesis of preeclampsia. Nat Med 2006, 12, 642-649.

Preliminary scope of work

1. Rešerše odborné literatury
2. Analyza vybranných markerů ve stěně cévy
3. Praktické provedení daných úkolů
4. Zhodnocení
5. Diskuse