Studium post translačních modifikací fosducinu

• Thesis title in Czech: Studium post translačních modifikací fosducinu
• Thesis title in English: Study of the posttrans lation al modifications of phosducin
• Key words: fosducin, SUMOylace, fosforylace, protein 14-3-3
• English key words: phosducin, SUMOylation, phosphorylation, protein 14-3-3
• Academic year of topic announcement: 2014/2015
• Thesis type: diploma thesis
• Thesis language: čeština
• Department: Department of Physical and Macromolecular Chemistry (31-260)
• Supervisor: prof. RNDr. Tomáš Obšil, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 27.11.2014
• Date of assignment: 27.11.2014
• Date of electronic submission: 09.05.2016
• Date of proceeded defence: 25.05.2016
• Opponents: RNDr. Miroslava Alblová, Ph.D.

Preliminary scope of work

Příprava fosducinu a proteinu 14-3-3 pomocí (exprese v bakteriích E. coli, purifikace pomocí chromatografických metod).
Fosforylace fosducinu pomocí PKA.
SUMOylace fosducinu (fosforylovaného i nefosforylovaného) a studium vlivu vazby proteinu 14-3-3 na tuto modifikaci.
Detekce SUMOylace technikou western blot.