Inhibiční studie lidské 17β-HSD3
| Thesis title in Czech: | Inhibiční studie lidské 17β-HSD3 |
|---|---|
| Thesis title in English: | Inhibition study of human 17β-HSD3 |
| Academic year of topic announcement: | 2013/2014 |
| Thesis type: | diploma thesis |
| Thesis language: | čeština |
| Department: | Department of Biochemical Sciences (16-16160) |
| Supervisor: | PharmDr. Hana Navrátilová, Ph.D. |
| Author: | hidden - assigned by the advisor |
| Date of registration: | 05.11.2013 |
| Date of assignment: | 24.02.2015 |
| Date and time of defence: | 04.06.2015 00:00 |
| Date of electronic submission: | 14.05.2015 |
| Date of proceeded defence: | 04.06.2015 |
| Opponents: | doc. RNDr. Lucie Zemanová, Ph.D. |
| Guidelines |
| Rešerše odborné literatury - lidské 17β-HSD u člověka, role v metabolismu steroidů, role v onemocněních, inhibitory
Osvojení si zakladních molekulárně biologických metod a klonování Příprava rekombinantní formy vybraného enzymu ze skupiny 17β-HSD Osvojení si práce s enzymovým vzorkem - inkubace 17β-HSD s vybraným substrátem se steroidní strukturou, stanovení enzymové aktivity Vyhodnocení získaných dat Zpracování rešerše a výsledků do konečné podoby diplomové práce Rozsah do 60 stran Rozsah grafické dokumentace: 5 obrázků, 5 tabulek, 2 grafy |
| References |
| Aka, J. A., Mazumdar, M., & Lin, S.-X. (2009). Reductive 17beta-hydroxysteroid dehydrogenases in the sulfatase pathway: critical in the cell proliferation of breast cancer. Molecular and cellular endocrinology, 301(1-2), 183–90.
Harada, K., Kubo, H., Tanaka, A., & Nishioka, K. (2012). Identification of oxazolidinediones and thiazolidinediones as potent 17β-hydroxysteroid dehydrogenase type 3 inhibitors. Bioorganic & medicinal chemistry letters, 22(1), 504–7. Legeza, B., Balázs, Z., Nashev, L. G., & Odermatt, A. (2013). The microsomal enzyme 17β-hydroxysteroid dehydrogenase 3 faces the cytoplasm and uses NADPH generated by glucose-6-phosphate dehydrogenase. Endocrinology, 154(1), 205–13. Lukacik, P., Kavanagh, K., & Oppermann, U. (2006). Structure and function of human 17beta-hydroxysteroid dehydrogenases. Molecular and cellular endocrinology, 248(1-2), 61–71. Marchais-Oberwinkler, S., Henn, C., Möller, G., Klein, T., Negri, M., Oster, A., … Adamski, J. (2011). 17β-Hydroxysteroid dehydrogenases (17β-HSDs) as therapeutic targets: protein structures, functions, and recent progress in inhibitor development. The Journal of steroid biochemistry and molecular biology, 125(1-2), 66–82. Payne, A. H., & Hales, D. B. (2004). Overview of steroidogenic enzymes in the pathway from cholesterol to active steroid hormones. Endocrine reviews, 25(6), 947–70. Törn, S., Nokelainen, P., Kurkela, R., Pulkka, A., Menjivar, M., Ghosh, S., … Vihko, P. (2003). Production, purification, and functional analysis of recombinant human and mouse 17β-hydroxysteroid dehydrogenase type 7. Biochemical and Biophysical Research Communications, 305(1), 37–45. |