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Thesis details
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"Studium lipoproteinů metodou SEC-LLSD"
Thesis title in Czech: "Studium lipoproteinů metodou SEC-LLSD"
Thesis title in English: Lipoprotein study by SEC/LLSD method
Key words: lipoproteins, SEC, LLSD
English key words: Lipoproteins, SEC, LLSD
Academic year of topic announcement: 2011/2012
Thesis type: diploma thesis
Thesis language: čeština
Department: Department of Analytical Chemistry (31-230)
Supervisor: RNDr. Eva Tvrzická, CSc.
Author: hidden - assigned by the advisor
Date of registration: 28.11.2011
Date of assignment: 16.01.2012
Date of electronic submission:23.05.2014
Date of proceeded defence: 03.06.2014
Opponents: Mgr. Barbora Staňková, Ph.D.
 
 
 
Advisors: prof. RNDr. Věra Pacáková, CSc.
Preliminary scope of work
Separace lipoproteinů ultracentrifugou; Gravimetrické stanovení koncentrace lipoproteinu (stanovení proteinu, cholesterolu a triglyceridu rutinní metodou); Určení závislosti dn/dc; Na základě přímé kalibrace a elučního času určení velikosti sledovaných částic; Srovnání chromatografického chování lipoproteinů na různých kolonách pro optimalizaci podmínek
Preliminary scope of work in English
Separation of lipoproteins by ultracentrifugation; Gravimetric determination of concentration of lipoproteins (determination of proteins, chloesterol and triglycerides by routine method); Determination of dn/dc dependences; Determination of particle size on the bases of direct calibration and elution times; Comparison of chromatographic behaviour of lipoproteins on various columns for optimalisation of experimental conditions.
 
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