Navození sporulace a tvorby pohlavního stádia u Ascomycota (Fungi)

• Thesis title in Czech: Navození sporulace a tvorby pohlavního stádia u Ascomycota (Fungi)
• Thesis title in English: Induction of sporulation and sexual state formation in Ascomycota (Fungi)
• Key words: fungi, sexual state, teleomorph, cultivations media, MAT factors
• English key words: houby, pohlavní stádium, teleomorfa, kultivační média, MAT faktory
• Academic year of topic announcement: 2010/2011
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Botany (31-120)
• Supervisor: Mgr. Miroslav Kolařík, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 22.11.2010
• Date of assignment: 17.12.2010
• Date of electronic submission: 09.05.2011
• Date of proceeded defence: 13.06.2011
• Opponents: RNDr. David Novotný, Ph.D.
• Advisors: RNDr. Alena Kubátová, CSc.

Preliminary scope of work

Cílem je
1) Podat přehled o mechanismech ovlivňujících sporulaci (tvorba nepohlavních konidií) a tvorbě pohlavního stádia u hub. Přehled by měly sloužit jako podklad pro chápání těchto procesů.
2) Podat přehled metodik (použití různých abiotických vlivů, chemických stimulantů, zdrojů živin, volba vhodných kmenů na pokusy) použitých pro stimulaci konidiace a produkce teloeomorfy. Přehled poslouží jako praktická pomůcka pří hledání vhodné metodiky indukce sledovaných fenoménů.
Tento přehled se pro zúžení bude zaměřovat na Askomycota. Pro inspiraci doporučiji nevyhýbat se ani zásadním pracím ?o zygo- a basiodiomycetech.
V práci by se měly objevit následující kapitoly:
1) faktory ovlivňující sporulaci: abiotické faktory (teplota, světelný režim atd.) a biotické faktory (voda, zdroje C, N, P, S, esenciální látky atd)
2) faktory ovlivňující tvorbu pohlavního stadia - abiotické faktory (teplota, světelný režim atd.) a biotické faktory (voda, zdroje C, N, P, S, esenciální látky atd)
3) kultivační média doporučovaná pro navození sporulace - přehled druhů a médií (tabulka)
4) kultivační média doporučovaná pro navození tvorby pohlavního stádia: přehled druhů a médií (tabulka)
5) Genetické pozadí tvorby sporulace
6) Genetické pozadí tvorby pohlavního stádia
Doporučená literatura
1.Turgeon, B.G., Application of mating type gene technology to problems in fungal biology. 1998. p. 115-137.
2.Sharon, A., et al., An asexual fungus has the potential for sexual development. Molecular and General Genetics, 1996. 251(1): p. 60-68.
3.Fleck, C.B. and M. Brock, Aspergillus fumigatus catalytic glucokinase and hexokinase: Expression analysis and importance for germination, growth, and conidiation. Eukaryotic Cell, 2010. 9(7): p. 1120-1135.
4.Honma, K. and K. Nishimoto, Attempt to make a suitable medium for induction of teleomorph of Arthroderma vanbreuseghemii. Japanese Journal of Medical Mycology, 1989. 30(3): p. 182-186.
5.Simkovič, M., et al., Ca2+-dependent induction of conidiation in submerged cultures of Trichoderma viride. Canadian Journal of Microbiology, 2008. 54(4): p. 291-298.
6.Zaffarano, P.L., A. Du?, and C.R. Grünig, Characterization of the mating type (MAT) locus in the Phialocephala fortinii s.l. - Acephala applanata species complex. Fungal Genetics and Biology, 2010. 47(9): p. 761-772.
7.Roncal, T. and U. Ugalde, Conidiation induction in Penicillium. Research in Microbiology, 2003. 154(8): p. 539-546.
8.Szewczyk, E. and S. Krappmann, Conserved regulators of mating are essential for Aspergillus fumigatus cleistothecium formation. Eukaryotic Cell, 2010. 9(5): p. 774-783.
9.Dyer, P.S., D.S. Ingram, and K. Johnstone, The control of sexual morphogenesis in the Ascomycotina. Biological Reviews of the Cambridge Philosophical Society, 1992. 67(4): p. 421-458.
10.Kück, U. and S. Pöggeler, Cryptic sex in fungi. Fungal Biology Reviews, 2009. 23(3): p. 86-90.
11.Nowrousian, M., et al., De novo assembly of a 40 Mb eukaryotic genome from short sequence reads: Sordaria macrospora, a model organism for fungal morphogenesis. PLoS Genetics, 2010. 6(4).
12.Singh, M.P.N., et al., Effect of glucose on growth, sporulation, folin-positive and protein production by blight pathogens. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 2005. 7(2): p. 289-292.
13.Gao, L., et al., Effects of carbon concentration and carbon to nitrogen ratio on the growth and sporulation of several biocontrol fungi. Mycological Research, 2007. 111(1): p. 87-92.
14.Lee, S.C., et al., The evolution of sex: A perspective from the fungal kingdom. Microbiology and Molecular Biology Reviews, 2010. 74(2): p. 298-340.
15.Lee, J., J.F. Leslie, and R.L. Bowden, Expression and function of sex pheromones and receptors in the homothallic ascomycete Gibberella zeae. Eukaryotic Cell, 2008. 7(7): p. 1211-1221.
16.Cuomo, C.A. and B.W. Birren, The fungal genome initiative and lessons learned from genome sequencing. 2010. p. 833-855.
17.Sherwood, R.K. and R.J. Bennett, Fungal meiosis and parasexual reproduction - lessons from pathogenic yeast. Current Opinion in Microbiology, 2009. 12(6): p. 599-607.
18.Bieszke, J.A., L. Li, and K.A. Borkovich, The fungal opsin gene nop-1 is negatively-regulated by a component of the blue light sensing pathway and influences conidiation-specific gene expression in Neurospora crassa. Current Genetics, 2007. 52(3-4): p. 149-157.
19.Casselton, L.A., Fungal sex genes - Searching for the ancestors. Bioessays, 2008. 30(8): p. 711-714.
20.Hornok, L., C. Waalwijk, and J.F. Leslie, Genetic factors affecting sexual reproduction in toxigenic Fusarium species. International Journal of Food Microbiology, 2007. 119(1-2): p. 54-58.
21.Posggeler, S., Genomic evidence for mating abilities in the asexual pathogen Aspergillus fumigatus. Current Genetics, 2002. 42(3): p. 153-160.
22.Singh, K.V. and S.C. Agrawal, Growth responses of keratinophilic fungi to some volatile substances. Mykosen, 1981. 24(10): p. 630-634.
23.Flaherty, J.E. and L.D. Dunkle, Identification and expression analysis of regulatory genes induced during conidiation in Exserohilum turcicum. Fungal Genetics and Biology, 2005. 42(5): p. 471-481.
24.El-Abyad, M.S., H. Hindorf, and M.A. Rizk, Impact of salinity stress on soil-borne fungi of sugarbeet - II. Growth activities in vitro. Plant and Soil, 1988. 110(1): p. 33-37.
25.Krasniewski, I., et al., Impact of solid medium composition on the conidiation in Penicillium camemberti. Process Biochemistry, 2006. 41(6): p. 1318-1324.
26.Szafranek, B., et al., In Vitro effects of cuticular lipids of the aphids Sitobion avenae, Hyalopterus pruni and Brevicoryne brassicae on growth and sporulation of the Paecilomyces fumosoroseus and Beauveria bassiana. Arkivoc, 2001. 2001(3): p. 81-94.
27.Nemčovič, M., et al., Induction of conidiation by endogenous volatile compounds in Trichoderma spp. FEMS Microbiology Letters, 2008. 284(2): p. 231-236.
28.Larmour, R. and R. Marchant, The induction of conidiation in Fusarium culmorum grown in continuous culture. Journal of General Microbiology, 1977. 99(1): p. 49-58.
29.Metz, A.M., et al., Induction of the sexual stage of Pestalotiopsis microspora, a taxol-producing fungus. Microbiology, 2000. 146(8): p. 2079-2089.
30.Zhou, T., R.D. Reeleder, and S.A. Sparace, Influence of nutrients on growth of Epicoccum nigrum. Canadian Journal of Microbiology, 1996. 42(7): p. 647-654.
31.Jacobsen, S., M. Wittig, and S. Pöggeler, Interaction between mating-type proteins from the homothallic fungus Sordaria macrospora. Current Genetics, 2002. 41(3): p. 150-158.
32.Steyaert, J.M., R.J. Weld, and A. Stewart, Isolate-specific conidiation in Trichoderma in response to different nitrogen sources. Fungal Biology, 2010. 114(2-3): p. 179-188.
33.Lauter, F.R., C.T. Yamashiro, and C. Yanofsky, Light stimulation of conidiation in Neurospora crassa: Studies with the wild-type strain and mutants wc-1, wc-2 and acon-2. Journal of Photochemistry and Photobiology B: Biology, 1997. 37(3): p. 203-211.
34.Seibel, C., et al., Light-dependent roles of the G-protein ? subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei). BMC Biology, 2009. 7: p. 58.
35.Tan, K.K., Light-induced synchronous conidiation in the fungus Botrytis cinerea. Journal of General Microbiology, 1976. 93(2): p. 278-282.
36.Sánchez-Murillo, R.I., et al., Light-regulated asexual reproduction Paecilomyces fumosoroseus. Microbiology, 2004. 150(2): p. 311-319.
37.Casselton, L.A., Mate recognition in fungi. Heredity, 2002. 88(2): p. 142-147.
38.Nelson, M.A., Mating systems in ascomycetes: A romp in the sac. Trends in Genetics, 1996. 12(2): p. 69-74.
39.Alvarez-Perez, S., et al., Mating type and invasiveness are significantly associated in Aspergillus fumigatus. Medical Mycology, 2010. 48(2): p. 273-277.
40.Glass, N.L. and G.A. Kuldau, Mating type and vegetative incompatibility in filamentous ascomycetes. Annual review of phytopathology. Vol. 30, 1992: p. 201-224.
41.Kronstad, J.W. and C. Staben, Mating type in filamentous fungi. 1997. p. 245-276.
42.Metin, B., K. Findley, and J. Heitman, The mating type locus (MAT) and sexual reproduction of Cryptococcus heveanensis: Insights into the evolution of sex and sex-determining chromosomal regions in fungi. PLoS Genetics, 2010. 6(5): p. 14.
43.Coppin, E., et al., Mating types and sexual development in filamentous ascomycetes. Microbiology and Molecular Biology Reviews, 1997. 61(4): p. 411-428.
44.Menkis, A., et al., The mating-type chromosome in the filamentous ascomycete Neurospora tetrasperma represents a model for early evolution of sex chromosomes. PLoS Genetics, 2008. 4(3).
45.Pöggeler, S., et al., Mating-type genes from the homothallic fungus Sordaria macrospora are functionally expressed in a heterothallic ascomycete. Genetics, 1997. 147(2): p. 567-580.
46.Liu, J., Y. Cao, and Y. Xia, Mmc, a gene involved in microcycle conidiation of the entomopathogenic fungus Metarhizium anisopliae. Journal of Invertebrate Pathology, 2010. 105(2): p. 132-138.
47.Hosid, E., et al., The mode of reproduction in natural populations of ascomycetous fungus, Emericella nidulans, from Israel. Genetics Research, 2010. 92(2): p. 83-90.
48.Casselton, L.A. and N.S. Olesnicky, Molecular genetics of mating recognition in basidiomycete fungi. Microbiology and Molecular Biology Reviews, 1998. 62(1): p. 55-70.
49.Esposito, M.S. and R.E. Esposito, Mutants of meiosis and ascospore formation. Methods in Cell Biology, 1975. vol.11: p. 303-326.
50.Gunnar K?lmark, H., Mutants with continuous microcycle conidiation in the filamentous fungus Fusarium solani f. sp. pisi. MGG Molecular & General Genetics, 1984. 198(1): p. 12-18.
51.Roncal, T., et al., Novel diterpenes with potent conidiation inducing activity. Tetrahedron Letters, 2002. 43(38): p. 6799-6802.
52.Gao, L. and X.Z. Liu, A novel two-stage cultivation method to optimize carbon concentration and carbon-to-nitrogen ratio for sporulation of biocontrol fungi. Folia Microbiologica, 2009. 54(2): p. 142-146.
53.Santos, J.M., V.G. Correia, and A.J.L. Phillips, Primers for mating-type diagnosis in Diaporthe and Phomopsis: their use in teleomorph induction in vitro and biological species definition. Fungal Biology, 2010. 114(2-3): p. 255-270.
54.Olmedo, M., C. Ruger-Herreros, and L.M. Corrochano, Regulation by blue light of the fluffy gene encoding a major regulator of conidiation in Neurospora crassa. Genetics, 2010. 184(3): p. 651-658.
55.Betina, V. and J. Zajacová, Regulation of periodicity and intensity of photo-induced conidiation of Trichoderma viride. Folia Microbiologica, 1978. 23(6): p. 453-459.
56.Steyaert, J.M., et al., Reproduction without sex: Conidiation in the filamentous fungus Trichoderma. Microbiology, 2010. 156(10): p. 2887-2900.
57.Tzima, A., et al., Roles of the catalytic subunit of cAMP-dependent protein kinase A in virulence and development of the soilborne plant pathogen Verticillium dahliae. Fungal Genetics and Biology, 2010. 47(5): p. 406-415.
58.Dunthorn, M. and L.A. Katz, Secretive ciliates and putative asexuality in microbial eukaryotes. Trends in Microbiology, 2010. 18(5): p. 183-188.
59.Ueng, P.P., et al., Sequence diversity of mating-type genes in Phaeosphaeria avenaria. Current Genetics, 2003. 43(2): p. 121-130.
60.Souza, C.A.J., C.C. Silva, and A.V.B. Ferreira, Sex in fungi: Lessons of gene regulation. Genetics and Molecular Research, 2003. 2(1): p. 136-147.
61.López-Villavicencio, M., et al., Sex in Penicillium: Combined phylogenetic and experimental approaches. Fungal Genetics and Biology, 2010. 47(8): p. 693-706.
62.Kothe, E., Sexual attraction: On the role of fungal pheromone/receptor systems (A review). Acta Microbiologica et Immunologica Hungarica, 2008. 55(2): p. 125-143.
63.Patel, P.V. and J.J. Miller, Stimulation of yeast sporulation by glycerol. Journal of Applied Bacteriology, 1972. 35(1): p. 63-69.
64.Schmoll, M., E.U. Esquivel-Naranjo, and A. Herrera-Estrella, Trichoderma in the light of day - Physiology and development. Fungal Genetics and Biology, 2010. 47(11): p. 909-916.
65.Gunasekaran, M. and D.J. Weber, Tryptophan stimulation of growth and sporulation of Rhizopus arrhizus Fischer. Canadian Journal of Microbiology, 1972. 18(8): p. 1185-1190.