α-N-Acetylgalactosaminidase as a tools in the synthesis of complex oligosaccharide immune stimulators

• Thesis title in Czech: Použití α-N-Acetylgalactosaminidasy jako nástroje vhodného pro syntézu komplexních oligosacharidových stimulátorů imunitního systému
• Thesis title in English: α-N-Acetylgalactosaminidase as a tools in the synthesis of complex oligosaccharide immune stimulators
• Academic year of topic announcement: 2009/2010
• Thesis type: dissertation
• Thesis language: angličtina
• Department: Department of Biochemistry (31-250)
• Supervisor: prof. RNDr. Karel Bezouška, DSc.
• Author: hidden - assigned by the advisor
• Date of registration: 01.10.2007
• Date of assignment: 01.10.2007
• Date of electronic submission: 09.08.2011
• Date of proceeded defence: 14.09.2011
• Opponents: prof. doc. Ing. Tomáš Macek, CSc.

Preliminary scope of work

Podařilo se připravit stabilní rekombinantní a-N-acetylgalaktosaminidasu expresí v S. cerevisiae, a studovat jjeí vlastnosti. Enzym může být potencionálně využitelný v biotechnologiích a při syntéze sacharidových mimetik.

Preliminary scope of work in English

In conclusion, I prepared stable, active recombinant α-NAGA in large quantities in a simple eukaryotic system of Saccharomyces cerevisiae. The notable advantage of our expression system is in shorter production times, and, up to fourfold increase of the enzyme yields compared to the native production system. Unique properties of this enzyme can find a use for the enzymatic synthesis of various carbohydrate structures and for transformation of the red blood cell group A to the group of H (0), the universal donor.