Mechanisms of membrane protein quality control in the endoplasmic reticulum

• Thesis title in Czech: Mechanismy kontroly kvality membránových proteinů v endoplasmatickém retikulu
• Thesis title in English: Mechanisms of membrane protein quality control in the endoplasmic reticulum
• Key words: membránový protein, kontrola kvality proteinů, translokace, sbalování, degradace, ERAD
• English key words: membrane protein, protein quality control, translocation, folding, degradation, ERAD
• Academic year of topic announcement: 2023/2024
• Thesis type: Bachelor's thesis
• Thesis language: angličtina
• Department: Department of Genetics and Microbiology (31-140)
• Supervisor: Ing. Kvido Stříšovský, Ph.D.
• Author: hidden - assigned and confirmed by the Study Dept.
• Date of registration: 22.12.2023
• Date of assignment: 22.12.2023
• Confirmed by Study dept. on: 31.01.2024
• Date of electronic submission: 30.04.2024
• Date of proceeded defence: 04.06.2024
• Opponents: RNDr. Cyril Bařinka, Ph.D.

Guidelines

Biologické membrány
Buněčná biologie

References

Christianson JC, Carvalho P (2022) Order through destruction: how ER-associated protein degradation contributes to organelle homeostasis. EMBO J: e109845
Christianson JC, Jarosch E, Sommer T (2023) Mechanisms of substrate processing during ER-associated protein degradation. Nat Rev Mol Cell Biol
Christianson JC, Ye Y (2014) Cleaning up in the endoplasmic reticulum: ubiquitin in charge. Nat Struct Mol Biol 21: 325-335
Hegde RS (2022) The Function, Structure, and Origins of the ER Membrane Protein Complex. Annu Rev Biochem
Hegde RS, Keenan RJ (2022) The mechanisms of integral membrane protein biogenesis. Nat Rev Mol Cell Biol 23: 107-124
Wu X, Rapoport TA (2021) Translocation of Proteins through a Distorted Lipid Bilayer. Trends Cell Biol
a clanky v odkazech

Preliminary scope of work

Mechanismy kontroly kvality membránových proteinů v endoplasmatickém retikulu s důrazem na mechanismy rozpoznání, retrotranslokace a degradace membránových proteinů. Student rekapituluje nové poznatky biogeneze membránových proteinů, jejich intramembránový folding, chaperony, rozpoznávání defektů foldingu a nové poznatky ohledně mechanismů degradace membránových proteinů s důrazem na ERAD.

Preliminary scope of work in English

Mechanisms of membrane protein quality control in the endoplasmic reticulum with emphasis on mechanisms of membrane protein recognition, retrotranslocation and degradation. The student will review new knowledge of membrane protein biogenesis, intramembrane protein folding, chaperones, recognition of folding defects, and new findings regarding mechanisms of membrane protein degradation with emphasis on ERAD.