DNA topologie jako faktor ovlivňující transkripci u bakterií

• Thesis title in Czech: DNA topologie jako faktor ovlivňující transkripci u bakterií
• Thesis title in English: DNA topology as a factor affecting transcription in bacteria
• Key words: DNA, topologie, RNA polymeráza, transkripce, promotor, faktor sigma
• English key words: DNA, topoogy, RNA polymerase, transcription, promoter, sigma factor
• Academic year of topic announcement: 2023/2024
• Thesis type: Bachelor's thesis
• Thesis language: čeština
• Department: Department of Genetics and Microbiology (31-140)
• Supervisor: doc. Mgr. Libor Krásný, Ph.D.
• Author: hidden - assigned and confirmed by the Study Dept.
• Date of registration: 17.10.2023
• Date of assignment: 17.10.2023
• Confirmed by Study dept. on: 25.01.2024
• Date of electronic submission: 29.04.2024
• Date of proceeded defence: 03.06.2024
• Opponents: RNDr. Jan Nešvera, CSc.

Preliminary scope of work

Rešeršní práce má za hlavní cíl zmapvat vliv topologie DNA na transkripci u bakterií. Tato problematika bude zasazena do širšího kontextu genové exprese. Student popíše klíčové hráče transkripce, jejich parametry a v detailu se soustředí na vliv stavu DNA šroubovice na regulaci transkripce, zejm. transkripční iniciace. Tato práce je motivována paralelním experimentálním projektem adresujícím toto téma, na kterém bude student pracovat.

Preliminary scope of work in English

The main aim of this Bachelor Thesis (BT) will be a review of the literature focusing on the effects of DNA topology on transcription in bacteria. This topic will be discussed in the wider context of gene expression. The student will describe key players of the process, their parameters, and will focus in detail on the effects of supercoiling on transcription, especially on transcription initiation. This BT will provide a background for a parallel experimental project of the student.