Migrace a patogenita schistosom v plicích myší a imunitní odpověď hostitele
| Thesis title in Czech: | Migrace a patogenita schistosom v plicích myší a imunitní odpověď hostitele |
|---|---|
| Thesis title in English: | Migration and pathogenicity of schistosomes in mouse lungs and the host immune response |
| Key words: | plíce, migrace, patogenita, Trichobilharzia, Schistosoma |
| English key words: | lungs, migration, pathogenicity, Trichobilharzia, Schistosoma |
| Academic year of topic announcement: | 2023/2024 |
| Thesis type: | diploma thesis |
| Thesis language: | čeština |
| Department: | Department of Parasitology (31-161) |
| Supervisor: | RNDr. Tomáš Macháček, Ph.D. |
| Author: | hidden - assigned and confirmed by the Study Dept. |
| Date of registration: | 18.10.2023 |
| Date of assignment: | 18.10.2023 |
| Confirmed by Study dept. on: | 03.01.2024 |
| Advisors: | Mgr. Martin Majer, Ph.D. |
| Guidelines |
| MB160P33 Biologie helmintů MB160P75E Parasite Immunology |
| References |
| Haas, W., & Pietsch, U. (1991). Migration of Trichobilharzia ocellata schistosomula in the duck and in the abnormal murine host. Parasitology Research, 77, 642-644. Horák, P., & Kolářová, L. (2000). Survival of bird schistosomes in mammalian lungs. International Journal for Parasitology, 30(1), 65-68. Houlder, E. L., Costain,A. H., Cook, P. C., & MacDonald, A. S. (2021). Schistosomes in the lung: immunobiology and opportunity. Frontiers in immunology, 12, 635513. Houlder, E. L., Costain, A. H., Nambuya, I., Brown, S. L., Koopman, J. P. R., Langenberg, M. C. C., ... & MacDonald, A. S. (2023). Pulmonary inflammation promoted by type-2 dendritic cells is a feature of human and murine schistosomiasis. Nature communications, 14(1), 1863. Chanová, M., Vuong, S., & Horák, P. (2007). Trichobilharzia szidati: the lung phase of migration within avian and mammalian hosts. Parasitology research, 100, 1243-1247. Kumar, R., Lee, M. H., Kassa, B., Fonseca Balladares, D. C., Mickael, C., Sanders, L., ... & Graham, B. B. (2023). Repetitive schistosoma exposure causes perivascular lung fibrosis and persistent pulmonary hypertension. Clinical Science, 137(8), 617-631. |
| Preliminary scope of work |
| Cílem práce je podrobně popsat migraci a patogenitu schistosom v plicích myší. Jako modelový druh bude zejména použita ptačí motolice Trichobilharzia szidati. S využitím řady komplementárních metod (histologie, imunofluorescence, průtoková cytometrie, restimulace leukocytů, ELISA aj.) bude v několika časových bodech studováno její chování v myších kmenů C57BL/6J a Balb/c. Jako komparativní model bude ve vybraných časových bodech použit lidský druh Schistosoma mansoni. |