Optimalizácia procesu lyofilizácie polymérnych nanosfér
| Thesis title in thesis language (Slovak): | Optimalizácia procesu lyofilizácie polymérnych nanosfér |
|---|---|
| Thesis title in Czech: | Optimalizace lyofilizačního procesu pro polymerní nanosféry |
| Thesis title in English: | Optimization of lyophilization process for polymeric nanospheres |
| Key words: | lyofilizace, kryoprotektiva, lyoprotektiva, nanočástice, PLGA |
| English key words: | lyophilization, cryoprotectants, lyoprotectants, nanoparticles, PLGA |
| Academic year of topic announcement: | 2023/2024 |
| Thesis type: | diploma thesis |
| Thesis language: | slovenština |
| Department: | Department of Pharmaceutical Technology (16-16210) |
| Supervisor: | PharmDr. Ondřej Holas, Ph.D. |
| Author: | hidden - assigned by the advisor |
| Date of registration: | 30.11.2023 |
| Date of assignment: | 07.03.2025 |
| Date and time of defence: | 30.05.2025 08:00 |
| Date of electronic submission: | 15.05.2025 |
| Date of proceeded defence: | 30.05.2025 |
| Opponents: | PharmDr. Barbora Boltnarová, Ph.D. |
| Advisors: | Mgr. Nancy Mrózková |
| Guidelines |
| 1. prostudování literatury 2. experimentální část zaměřená na lyofilizaci 3. vyhodnocení výsledků 4. sepsání diplomové práce |
| References |
| Fonte P., Reis S., Sarmento B. Facts and evidences on the lyophilization of polymeric nanoparticles for drug delivery. J. Control. Release, 2016, 225, 75-86 Abdelwahed W., Degobert G., Stainmesse S., Fessi H. Freeze-drying of nanoparticles: Formulation, process and storage considerations. Adv. Drug Deliv. Rev. 2006, 58, 1688-1733 Bjelošević, M., et al., Excipients in freeze-dried biopharmaceuticals: Contributions toward formulation stability and lyophilisation cycle optimisation. Int J Pharm, 2020, 576, 119029. Holm, T.P., et al., Screening of novel excipients for freeze-dried protein formulations. Eur J Pharm Biopharm, 2021, 160, 55-64. Holzer, M., et al., Physico-chemical characterisation of PLGA nanoparticles after freeze-drying and storage, Eur J Pharm Biopharm, 2009. 72, 428-37. |
| Preliminary scope of work |
| 1. Seznámení se s problematikou lyofilizace 2. Příprava nanočástic s účinnou látkou 3. Volba Vhodných pomocných látek pro lyofilizaci 4. Optimalizace paramterů lyofilzace 5. Vyhodnocení parametrů nanočástic před a po lyofilizaci |
| Preliminary scope of work in English |
| 1. Introduction to lyophilization 2. Preparation of nanoparticles with various stabilizers 3. Selection of suitable excipients for lyophilization 4. Optimization of lyophilization parameters 5. Parameteres pre and post-lyophilization parameters |