Základní in vitro testování stříbrných nanočástic

• Thesis title in Czech: Základní in vitro testování stříbrných nanočástic
• Thesis title in English: Basic in vitro evaluation of silver nanoparticles
• Key words: stříbrné nanočástice, MIC, cytotoxicita, intracelulární bakterie, makrofágy
• English key words: silver nanoparticles, MIC, cytotoxicity, intracellular bacteria, macrophages
• Academic year of topic announcement: 2022/2023
• Thesis type: diploma thesis
• Thesis language: čeština
• Department: Department of Pharmaceutical Technology (16-16210)
• Supervisor: PharmDr. Ondřej Holas, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 29.09.2022
• Date of assignment: 14.12.2022
• Date and time of defence: 04.06.2024 12:45
• Date of electronic submission: 14.05.2024
• Date of proceeded defence: 04.06.2024
• Opponents: Mgr. Monika Smékalová, Ph.D.
• Advisors: doc. PharmDr. Ivona Pávková, Ph.D.

Guidelines

Student bude vykonávat experimentální práci na Katedře molekulární biologie a patologie Fakulty vojenského zdravotnictví Univerzity obrany.

References

- McLendon MK, Apicella MA, Allen LA. Francisella tularensis: taxonomy, genetics, and Immunopathogenesis of a potential agent of biowarfare. Annu Rev Microbiol. 2006;60:167-85. doi: 10.1146/annurev.micro.60.080805.142126. PMID: 16704343; PMCID: PMC1945232.
- Tärnvik A, Berglund L. Tularaemia. Eur Respir J. 2003 Feb;21(2):361-73. doi: 10.1183/09031936.03.00088903. PMID: 12608453.
- Sutera V, Caspar Y, Boisset S, Maurin M. A new dye uptake assay to test the activity of antibiotics against intracellular Francisella tularensis. Front Cell Infect Microbiol. 2014 Mar 18;4:36. doi: 10.3389/fcimb.2014.00036. PMID: 24672776; PMCID: PMC3957058.
- Georgi E, Schacht E, Scholz HC, Splettstoesser WD. Standardized broth microdilution antimicrobial susceptibility testing of Francisella tularensis subsp. holarctica strains from Europe and rare Francisella species. J Antimicrob Chemother. 2012 Oct;67(10):2429-33. doi: 10.1093/jac/dks238. Epub 2012 Jul 4. PMID: 22763567.
- Brown SD, Krisher K, Traczewski MM. Broth microdilution susceptibility testing of Francisella tularensis: quality control limits for nine antimicrobial agents and three standard quality control strains. J Clin Microbiol. 2004 Dec;42(12):5877-80. doi: 10.1128/JCM.42.12.5877-5880.2004. PMID: 15583330; PMCID: PMC535293.
- Dos Santos CA, Seckler MM, Ingle AP, Gupta I, Galdiero S, Galdiero M, Gade A, Rai M. Silver nanoparticles: therapeutical uses, toxicity, and safety issues. J Pharm Sci. 2014 Jul;103(7):1931-1944. doi: 10.1002/jps.24001. Epub 2014 May 13. PMID: 24824033.- Bruna - T, Maldonado-Bravo F, Jara P, Caro N. Silver Nanoparticles and Their Antibacterial Applications. Int J Mol Sci. 2021 Jul 4;22(13):7202. doi: 10.3390/ijms22137202. PMID: 34281254; PMCID: PMC8268496.

Preliminary scope of work

Seznámení studenta s literaturou a literárními zdroji. Zaškolení do in vitro metodik pro testování základních vlastností stříbrných nanočástic. Měření minimální inhibiční koncentrace (MIC). Vliv různých typů částic na hodnotu MIC. Stanovení buněčné cytotoxicity.

Preliminary scope of work in English

Acquaintance of the student with literature and literary sources. Training in in vitro methodologies for testing the basic properties of silver nanoparticles. Measurement of minimum inhibitory concentration (MIC). Effect of different types of particles on the MIC value. Determination of cellular cytotoxicity.