Vliv endoglinu na aktivaci jaterní fibrózy in vitro

• Thesis title in Czech: Vliv endoglinu na aktivaci jaterní fibrózy in vitro
• Thesis title in English: Role of endoglin in the activation of liver fibrosis in vitro
• Key words: endoglin, buněčné a tkáňové linie, farmakologicky aktivní látka
• English key words: endoglin, cell line, pharmacologically active substance
• Academic year of topic announcement: 2022/2023
• Thesis type: diploma thesis
• Thesis language: čeština
• Department: Department of Biological and Medical Sciences (16-16150)
• Supervisor: PharmDr. Petra Fikrová, Ph.D.
• Author: hidden - assigned by the advisor
• Date of registration: 27.09.2022
• Date of assignment: 27.02.2024
• Date and time of defence: 12.09.2024 08:00
• Date of electronic submission: 31.08.2024
• Date of proceeded defence: 12.09.2024
• Opponents: PharmDr. Katarína Tripská, Ph.D.

Guidelines

Postup při vypracování práce:
1. Literární rešerše odborné literatury
2. Praktická část - práce s buněčnými liniemi in vitro - RT-PCR, ELISA, WesternBlot
3. Statistické zpracování a hodnocení dat
5. Diskuze
6. Závěr

References

Botella, L. M., Sanchez-Elsner, T., Sanz-Rodriguez, F., Kojima, S., Shimada, J., Guerrero-Esteo, M., Cooreman, M. P., Ratziu, V., Langa, C., Vary, C. P., Ramirez, J. R., Friedman, S., and Bernabeu, C. (2002) Transcriptional activation of endoglin and transforming growth factor-beta signaling components by cooperative interaction between Sp1 and KLF6: their potential role in the response to vascular injury. Blood 100, 4001-4010
Gallardo-Vara, E., Blanco, F. J., Roque, M., Friedman, S. L., Suzuki, T., Botella, L. M., and Bernabeu, C. (2016) Transcription factor KLF6 upregulates expression of metalloprotease MMP14 and subsequent release of soluble endoglin during vascular injury. Angiogenesis 19, 155-171

Lawrence, T. (2009) The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol 1, a001651
Nachtigal, P., Zemankova Vecerova, L., Rathouska, J., and Strasky, Z. (2012) The role of endoglin in atherosclerosis. Atherosclerosis 224, 4-11
Rossi, E., Sanz-Rodriguez, F., Eleno, N., Duwell, A., Blanco, F. J., Langa, C., Botella, L. M., Cabanas, C., Lopez-Novoa, J. M., and Bernabeu, C. (2013) Endothelial endoglin is involved in inflammation: role in leukocyte adhesion and transmigration. Blood 121, 403-415
Rossi, E., Pericacho, M., Bachelot-Loza, C., Pidard, D., Gaussem, P., Poirault-Chassac, S., Blanco, F. J., Langa, C., Gonzalez-Manchon, C., Novoa, J. M. L., Smadja, D. M., and Bernabeu, C. (2018) Human endoglin as a potential new partner involved in platelet-endothelium interactions. Cell Mol Life Sci 75, 1269-1284
Zemankova, L., Varejckova, M., Dolezalova, E., Fikrova, P., Jezkova, K., Rathouska, J., Cerveny, L., Botella, L. M., Bernabeu, C., Nemeckova, I., and Nachtigal, P. (2015) Atorvastatin-induced endothelial nitric oxide synthase expression in endothelial cells is mediated by endoglin. J Physiol Pharmacol 66, 403-413